• Species Reactivity

  Human

• Specificity

  Detects human Claudin‑8. Stains human Claudin-8 transfectants but not irrelevant transfectants.

• Source

  Monoclonal Mouse IgG1 Clone # 474210

• Immunogen

  HEK293 human embryonic kidney cell line transfected with human Claudin-8    
  Met1-Val225 (Ser151Pro)    
  Accession # P56748

• Formulation

  Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

• Label

  Alexa Fluor 405

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Intracellular Staining by Flow Cytometry

  0.25-1 µg/10    ⁶ cells

  HEK293 human embryonic kidney cell line transfected with human Claudin-8

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Claudin-8

Claudin-8 is a 225 amino acid (aa), 25 kDa four-transmembrane protein in the Claudin family of tight junction proteins. It is selectively expressed in tight junctions in the kidney thin descending limb, distal tubule and collecting duct, but is also found in liver and intestinal tight junctions. It is thought to function as an ion barrier protein. The extracellular portions of human Claudin-8 share 86% and 89% aa identity with corresponding portions of mouse and rat Claudin-8, respectively.

• Entrez Gene IDs:

  9073 (Human); 54420 (Mouse); 304124 (Rat)

• Alternate Names:

  claudin 8; Claudin8; Claudin-8; CLDN8; human CLDN8 gene for claudin-810claudin-8