• Species Reactivity

  Human

• Specificity

  Detects human GPR22. Stains human GPR22 transfectants but not irrelevant transfectants.

• Source

  Monoclonal Mouse IgG2a Clone # 455108

• Immunogen

  HEK293 human embryonic kidney cell line transfected with human GPR22    
  Met1-Asp433    
  Accession # Q99680

• Formulation

  Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

• Label

  Alexa Fluor 750

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Intracellular Staining by Flow Cytometry

  0.25-1 µg/10    ⁶ cells

  A172 human glioblastoma cell line fixed with paraformaldehyde and permeabilized with saponin

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: GPR22

GPR22 is a 7TM G protein-coupled receptor that is expressed in the brain. It maps to chromosome 7, region q22-q31.1. Human GPR22 shares 97% aa sequence identity with mouse GPR22.

• Long Name:

  G Protein-coupled Receptor 22

• Entrez Gene IDs:

  2845 (Human); 73010 (Mouse); 298944 (Rat)

• Alternate Names:

  G protein-coupled receptor 22; GPR22; MGC129847; probable G-protein coupled receptor 22