• Species Reactivity

  Human

• Specificity

  Detects human EMP. Stains human EMP3 cell transfectants but not irrelevant transfectants.

• Source

  Monoclonal Mouse IgG2b Clone # 439949

• Immunogen

  NS0 mouse myeloma cell line transfected with human EMP3    
  Met1-Glu163    
  Accession # P54852

• Formulation

  Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

• Label

  Alexa Fluor 750

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Intracellular Staining by Flow Cytometry

  0.25-1 µg/10    ⁶ cells

  Human peripheral blood mononuclear cells fixed with paraformaldehyde and permeabilized with saponin

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: EMP3

Epithelial membrane protein 3 (EMP3) is a multipass transmembrane protein in the peripheral myelin protein 22 family. It is expressed as a 20‑25 kDa molecule depending on the degree of glycosylation. EMP3 interacts with the P2X7 purinergic receptor on monocytes. Its dysregulation is associated with glioma and neuroblastoma. Human EMP3 shares 93% aa sequence identity with mouse and rat EMP3.

• Long Name:

  Epithelial Membrane Protein 3

• Entrez Gene IDs:

  2014 (Human); 13732 (Mouse); 81505 (Rat)

• Alternate Names:

  EMP3; EMP-3; epithelial membrane protein 3; HNMP-1; Protein YMP; YMP; YMPHematopoietic neural membrane protein 1