• Species Reactivity

  Human

• Specificity

  Detects human Aldehyde Dehydrogenase 1-A1/ALDH1-A1 in direct ELISAs and Western blots. In direct ELISAs, approximatly    
  50% cross‑reactivity with recombinant human Aldehyde Dehydrogenase 1-A2 is observed.

• Source

  Monoclonal Mouse IgG    _2B Clone # 703410

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  E. coli-derived recombinant human Aldehyde Dehydrogenase 1-A1/ALDH1-A1    
  Ser2-Ser501    
  Accession # P00352

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Western Blot

  2 µg/mL

  See below

• 
  

• Simple Western

  20 µg/mL

  See below

• 
  

• Immunohistochemistry

  8-25 µg/mL

  See below

• 
  

• Immunocytochemistry

  8-25 µg/mL

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Sterile PBS to a final concentration of 0.5 mg/mL.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Aldehyde Dehydrogenase 1-A1/ALDH1A1

Aldehyde dehydrogenases (ALDHs) are NAD(P)⁺-dependent enzymes that detoxify aldehydes by oxidizing them to carboxylic acids. Nineteen ALDHs are present in humans, expressed in a variety of organelles and having different substrate preferences (1). ALDH1A1 is a cytosolic enzyme that preferentially oxidizes retinaldehyde to retinoic acid (2). ALDH1A1 is expressed in the epithelium of many organs, including brain, liver, testis, eye lens and cornea (3). ALDH1A1 is highly expressed in brain dopaminergic neurons, where it produces the retinoic acid required for their differentiation and development (4). The retinoic acid produced by ALDH1A1 is also important for the differentiation of hematopoietic stem cells (5). ALDH1A1 is a major enzyme in the oxidation of acetaldehyde, a toxic metabolite of ethanol (6).

• References:

1. (2008) Expert Opin. Drug Metab. Toxicol. 4:697.

2. Zhao, D. et al. (1996) Eur. J. Biochem. 240:15.

3. King, G. and Holmes, R. (1997) Adv. Exp. Med. Biol. 414:19.

4. Jacobs, F.M. et al. (2007) Development 134:2673.

5. Chute, J.P. et al. (2006) Proc. Natl. Acad. Sci. USA. 103:11707.

6. Ueshima, Y. et al. (1993) Alcohol Alcohol. 1B:15.

• Entrez Gene IDs:

  216 (Human)

• Alternate Names:

  acetaldehyde dehydrogenase 1; ALDC; aldehyde dehydrogenase 1 family, member A1; aldehyde dehydrogenase 1, soluble; Aldehyde Dehydrogenase 1A1; Aldehyde Dehydrogenase 1-A1; Aldehyde dehydrogenase family 1 member A1; Aldehyde dehydrogenase, cytosolic; aldehyde dehydrogenase, liver cytosolic; ALDH class 1; ALDH1; ALDH11; ALDH1A1; ALDH-E1; ALHDII; EC 1.2.1; EC 1.2.1.36; MGC2318; PUMB1; RALDH 1; RALDH1; retinal dehydrogenase 1; retinaldehyde dehydrogenase 1