• Species Reactivity

  Human

• Specificity

  Detects human VSIG1 in ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse VSIG1 or recombinant human VSIG2, 3, or 4 is observed.

• Source

  Monoclonal Mouse IgG    _2B Clone # 771506

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant human VSIG1    
  Val22-Gly234    
  Accession # Q86XK7

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Western Blot

  2 µg/mL

  See below

• 
  

• Simple Western

  100 µg/mL

  See below

• 
  

• Immunohistochemistry

  8-25 µg/mL

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Sterile PBS to a final concentration of 0.5 mg/mL.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: VSIG1

VSIG1 (V-set and Ig domain-containing protein 1; also Glycoprotein A34) is a variably glycosylated 55‑70 kDa member of the JAM family of proteins. It has restricted expression, being limited to testicular germ cells plus pancreatic duct and gastric epithelium. VSIG1 is likely to serve as an adhesion molecule. Mature human VSIG1 is 366 amino acids (aa) in length. It is a type I transmembrane glycoprotein that contains a 211 aa extracellular domain (ECD). The ECD contains one V-type (aa 22‑132) and one C2-type Ig-like domain (aa 140‑227). Over aa 22‑234, human VSIG1 is 83% aa identical to both mouse and canine VSIG1. At least one potential splice variant exists in human. It shows an insertion of 36 aa after Ser72 and a deletion of aa 133‑387.

• Long Name:

  V-Set and Immunoglobulin Domain Containing 1

• Entrez Gene IDs:

  340547 (Human); 78789 (Mouse); 315920 (Rat)

• Alternate Names:

  Cell surface A33 antigen; dJ889N15.1; Glycoprotein A34; GPA34; MGC44287,1700062D20Rik; V-set and immunoglobulin domain containing 1; V-set and immunoglobulin domain-containing protein 1; VSIG1