• Species Reactivity

  Human

• Specificity

  Recognizes human Leukotriene B4 R1 (Pettersson, A.     et al. (2000) Biochem. Biophys. Res. Commun.     279:520).

• Source

  Monoclonal Mouse IgG1 Clone # 203/14F11

• Immunogen

  HeLa cervical epithelial carcinoma cell line transfected with human Leukotriene B4 R1

• Formulation

  Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

• Label

  Alexa Fluor 750

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Flow Cytometry

  0.25-1 µg/10    ⁶ cells

  Human peripheral blood monocytes

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Leukotriene B4 R1

Polymorphonuclear granulocytes secrete the lipid chemotactic mediator Leukotriene B4 (LTB4) in response to inflammatory stimuli (1). Neutrophils, monocytes and lymphocytes respond to LTB4 via specific receptors localized on the cell surface (2-4). The high affinity LTB4 Receptor known as BLT1 is only expressed on leukocytes (5-7) while a second low affinity receptor BLT2 is expressed more ubiquitously (8, 9). The BLT1 and BLT2 are G-protein linked seven-transmembrane spanning receptors that share about 37-45% amino acid identity (8, 9).  

• References:

1. Samuelsson, B. et al. (1987) Science 237:1171.

2. Brom, J. and W. Konig (1989) Immunology 68:479.

3. Patry, C. et al. (1996) Prostaglandins, Leukotrienes and Essential Fatty Acids 54:361.

4. Dasari, V.R. et al. (2000) Immunopharm. 48:157.

5. Pettersson, A. et al. (2000) Biochem. Biophys. Res. Comm. 279:520.

6. Yokomizo, T. et al. (1997) Nature 387:620.

7. Kato, K. et al. (2000) J. Exp. Med. 192:413.

8. Yokomizo, T. et al. (2000) J. Exp. Med. 192:421.

9. Kamohara, M. et al. (2000) J. Biol. Chem. 275:27000.

• Long Name:

  Leukotriene B4 Receptor 1

• Entrez Gene IDs:

  1241 (Human)

• Alternate Names:

  BLT1; chemokine receptor-like 1; CMKRL1; Leukotriene B4 R1; leukotriene B4 receptor; Leukotriene B4R1; LTB4R; LTB4R1; LTBR1; P2Y purinoceptor 7; purinergic receptor P2Y, G-protein coupled, 7