• Species Reactivity

  Human

• Specificity

  Detects human Erythropoietin R in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant mouse Erythropoietin R is observed.

• Source

  Monoclonal Mouse IgG    _2B Clone # 38409

• Purification

  Protein A or G purified from ascites

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant human Erythropoietin R    
  Pro26-Pro250    
  Accession # P19235

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Endotoxin Level

  <0.10 EU per 1 μg of the antibody by the LAL method.  

• Label

  Unconjugated

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Western Blot

  2 µg/mL

  See below

• 
  

• Immunoprecipitation

  Khankin, E.V.     et al. (2010) PLoS One     5: e9246.

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Erythropoietin R

Epo R is a transmembrane protein expressed on the surface of megakaryocytes, erythroid progenitors, and endothelial cells. It binds Epo and transmits signals that stimulate the proliferation and maturation of bone marrow erythroid precursors into red cells.

• Long Name:

  Erythropoietin Receptor

• Entrez Gene IDs:

  2057 (Human); 13857 (Mouse)

• Alternate Names:

  EpoR; EPO-R; Erythropoietin R; erythropoietin receptor; MGC138358