• Species Reactivity

  Human, Primate

• Specificity

  Detects human and primate Chitinase 3-like 1 in direct ELISAs.

• Source

  Monoclonal Rat IgG    ₁ Clone # 384327

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant human Chitinase 3-like 1    
  Tyr22-Thr383    
  Accession # P36222

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Human/Primate Chitinase 3-like 1 Sandwich Immunoassay

  Reagent    

• ELISA Capture (Matched Antibody Pair)

  2-8 µg/mL 

  Human/Primate Chitinase 3‑like 1 Antibody (Catalog # )

• ELISA Detection (Matched Antibody Pair)

  0.1-0.4 µg/mL 

  Human/Primate Chitinase 3‑like 1 Biotinylated Antibody (Catalog # )

• ELISA Standard

   

  Recombinant Human Chitinase 3-like 1 Protein, CF (Catalog # )

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Reconstitution

  Sterile PBS to a final concentration of 0.5 mg/mL.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Chitinase 3-like 1

Chitinase 3-like 1 (CHI3L1), also called YKL-40 or human cartilage glycoprotein 39 (HCGP39), is a 39 kDa glycoprotein member of the glycosyl hydrolase 18 family (1‑4). CHI3L1 was first identified as secreted from cultured articular chondrocytes, synovial cells, and activated monocyte‑derived macrophages, but it is also secreted by neutrophils, endothelial cells, vascular smooth muscle cells, and some cancer cells (1, 2, 5‑7). The human CHI3L1 cDNA encodes 383 amino acids (aa), including a 21 aa signal sequence and a 362 aa mature region with two intramolecular disulfides (3). Mature human CHI3L1 shares 73%, 80%, 82%, 83%, 86% and 87% aa sequence identity with mouse, rat, canine, bovine, porcine and equine CHI3L1, respectively. Human CHI3L1 and CHI3L2 share 43% aa sequence identity. Neither shows chitotriosidase activity, but both bind chitin and are thus termed chi-lectins (1‑4). CHI3L1 can bind heparins, probably as heparan sulfate (3, 7). It has been found to enhance cell adhesion and promote cell signaling, proliferation and tumor angiogenesis (4, 6‑9). Elevated serum CHI3L1 levels occur in some conditions characterized by inflammation and connective tissue remodeling, such as arthritis, chronic obstructive pulmonary disease, diabetes, cardiovascular disease, inflammatory bowel disease, and liver cirrhosis (1, 9‑12). Single nucleotide polymorphisms that can increase serum CHI3L1 are associated with higher risk for asthma in childhood (13). CHI3L1 is frequently up‑regulated in glioblastoma, myxoid chondrosarcoma, melanoma and carcinomas of the breast, thyroid, colon, lung, kidney, and ovary (9). In asthma and cancer, serum CHI3L1 concentrations can correlate with prognosis (9, 14, 15).

• References:

1. Hakala, B.E. et al. (1993) J. Biol. Chem. 268:25803.

2. Renkema, G.H. et al. (1998) Eur. J. Biochem. 251:504.

3. Fusetti, F. et al. (2003) J. Biol. Chem. 278:37753.

4. Houston, D.R. et al. (2003) J. Biol. Chem. 278:30206.

5. Volck, B. et al. (1998) Proc. Assoc. Am. Physicians 110:351.

6. Nishikawa, K.C. and A.J.T. Millis (2003) Exp. Cell Res. 287:79.

7. Shao, R. et al. (2009) Oncogene 28:4456.

8. Recklies, A.D. et al. (2002) Biochem. J. 365:119.

9. Eurich, K. et al. (2009) World J. Gastroenterol. 15:5249.

10. Letuve, S. et al. (2008) J Immunol. 181:5167.

11. Rathke, C.N. and H. Vestergaard (2009) Cardiovasc. Diabetol. 8:61.

12. Johansen, J.S. et al. (1997) Scand. J. Gastroenterol. 32:582.

13. Ober, C. et al. (2008) N. Engl. J. Med. 358:1682.

14. Johansen, J.S. et al. (1999) Rheumatology 38:618.

15. Chupp, G.L. et al. (2007) N. Engl. J. Med. 357:2016.

• Entrez Gene IDs:

  1116 (Human); 12654 (Mouse)

• Alternate Names:

  AW208766; Brp39; CHI3L1; Chitinase 3 like 1; Chitinase 3-like 1; gp39; HCgp39; YKL-40