• Species Reactivity

  Human

• Specificity

  Detects human RBP4/Retinol‑Binding Protein 4 in ELISAs. In sandwich immunoassays, no cross-reactivity with recombinant mouse RBP4 is observed.

• Source

  Monoclonal Mouse IgG    ₁ Clone # 393006

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant human RBP4/Retinol‑Binding Protein 4    
  Glu19-Leu201    
  Accession # P02753

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Human RBP4/Retinol Binding Protein 4 Sandwich Immunoassay

  Reagent    

• ELISA Capture (Matched Antibody Pair)

  2-8 µg/mL 

  Human RBP4/Retinol‑Binding Protein 4 Antibody (Catalog # )

• ELISA Detection (Matched Antibody Pair)

  0.5-2.0 µg/mL 

  Human RBP4/Retinol‑Binding Protein 4 Biotinylated Antibody (Catalog # )

• ELISA Standard

   

  Recombinant Human RBP4 Protein, CF (Catalog # )

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Reconstitution

  Sterile PBS to a final concentration of 0.5 mg/mL.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: RBP4/Retinol-Binding Protein 4

Retinol (also known as vitamin A) is unstable and insoluble in the aqueous solution (1, 2). However, retinol becomes quite stable and soluble in plasma due to its tight interaction with retinol-binding protein 4 (RBP4), also known as plasma retinol-binding protein. A prototypic member of the lipocalin superfamily, RBP4 has a beta -barrel structure with a well-defined cavity. It is secreted from the liver, a process requiring the availability of retinol. RBP4 delivers retinol from the liver to the peripheral tissues. In plasma, the RBP4-retinol complex interacts with transthyretin (TTR), also known as thyroxine-binding protein and prealbumin. The retinol-RBP4-TTR complex prevents the loss of RBP4 by filtration through the kidney and increases the stability of the retinol-RBP4 complex. Defects in RBP4 cause retinol-binding protein deficiency, which affects night vision. Human and mouse RBP4 share 86% amino acid sequence identity.

• References:

1. Zanotti, G. and R. Berni. (2004) Vitamins and Hormones 69:271.

2. Newcomer, M.E. and D.E. Ong. (2000) Biochim. Biophys. Acta 1482:57.

• Entrez Gene IDs:

  5950 (Human); 19662 (Mouse); 25703 (Rat)

• Alternate Names:

  interstitial; Plasma retinol-binding protein; RBP4; retinol binding protein 4, plasma; RetinolBinding Protein 4; retinol-binding protein 4, plasma