• Species Reactivity

  Human

• Specificity

  Detects human Osteoadherin/OSAD in ELISA.

• Source

  Monoclonal Mouse IgG    _2B Clone # 806001

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  HEK293 human embryonic kidney cell line transfected with human Osteoadherin/OSAD    
  Gln21-Gln421    
  Accession # Q99983

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Immunocytochemistry

  8-25 µg/mL

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Osteoadherin/OSAD

Osteoadherin (OSAD), also known as Osteomodulin, is an extracellular matrix keratan sulfate proteoglycan that belongs to the class II subfamily of small leucine-rich proteoglycans (SLRP). LRR motifs consist of approximately 20‑30 amino acids (aa) with conserved leucine spacing, folded into a structure with one beta -sheet and one alpha -helix (1, 2). The human OSAD cDNA encodes a 421 aa precursor that contains a 20 aa signal sequence and eleven tandem leucine rich repeats (3). Human OSAD shares 80‑84% aa sequence identity with bovine, mouse, and rat OSAD. Human OSAD shares 32‑35% aa sequence identity with human class II SLRPs Fibromodulin, Keratocan, Lumican, and PRELP. Bovine, mouse, and rat OSAD are expressed as 60‑85 kDa molecules, although the amino acid sequence for each predicts a size of 46‑47 kDa. The primary difference is due to the presence of extensive N-linked glycosylation that can also vary between tissues of the same species (4, 5). Human OSAD is expressed as an even larger 110 kDa molecule in teeth (6). OSAD contains eight sulfated tyrosine residues (4, 7) and is distinguished from other class II SLRPs by the presence of an approximately 70 aa C-terminal acidic domain (3). OSAD is expressed by fetal and adult osteoblasts but is not detectable in cartilage or tendon (3, 4, 8). In dental tissue, OSAD is expressed by odontoblasts and ameloblasts (5, 9‑11) and is involved in the mineralization of bone and teeth (5, 11, 12). OSAD promotes the adhesion of osteoblasts and odontoblasts to the surrounding matrix, an interaction that is mediated by Integrin alpha V beta 3 (4, 6).

• References:

1. Matsushima, N. et al. (2000) Proteins 38:210. 

2. Kobe, B. and A.V. Kajava (2001) Curr. Opin. Struct. Biol. 11:725. 

3. Sommarin, Y. et al. (1998) J. Biol. Chem. 273:16723. 

4. Wendel, M. et al. (1998) J. Cell Biol. 141:839. 

5. Hultenby, P.U. et al. (2003) Eur. J. Oral Sci. 111:128. 

6. Lucchini, M. et al. (2004) J. Dent. Res. 83:552. 

7. Onnerfjord, P. et al. (2004) J. Biol. Chem. 279:26

8. Shen, Z. et al. (1999) Matrix Biol. 18:533.

9. Buchaille, R. et al. (2000) Bone 27:265.

10. Buchaille, R. et al. (2000) Matrix Biol. 19:421.

11. Couble, M.L. et al. (2004) Histochem. Cell Biol. 121:47.

12. Ramstad, V.E. et al. (2003) Calcif. Tissue Int. 72:57.

• Entrez Gene IDs:

  4958 (Human)

• Alternate Names:

  Keratan sulfate proteoglycan osteomodulin; KSPG osteomodulin; OMD; OSAD; osteoadherin proteoglycan; Osteoadherin; osteomodulin; SLRR2C; SLRR2Costeoadherin