• Species Reactivity

  Human, Mouse

• Specificity

  Detects human Snapin in direct ELISAs and human and mouse Snapin in Western blots.

• Source

  Monoclonal Mouse IgG    _2A Clone # 809116

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  E. coli-derived recombinant human Snapin    
  Met1-Lys136    
  Accession # O95295

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Western Blot

  2 µg/mL

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Sterile PBS to a final concentration of 0.5 mg/mL.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Snapin

Snapin (SNARE-associated protein) is an approximately 15 kDa, 136 amino acid vesicular phosphoprotein that contains a coiled-coil interacting domain. In neurons, it binds SNAP25 and RGS7 and regulates steps between synaptic vesicle docking and neurotransmitter release. In pancreatic beta cells, it mediates incretin-stimulated insulin secretion. In germ cells, it binds PUM2 and Nanos1 as a putative adaptor protein. Human Snapin shares 98% aa sequence identity with mouse and rat Snapin, while mouse and rat Snapin share 100% aa sequence identity with each other.

• Long Name:

  SNAP-associated Protein

• Entrez Gene IDs:

  23557 (Human); 20615 (Mouse)

• Alternate Names:

  SNAP-25-binding protein; SNAP25BP; SNAPAP; SNAPAPSNARE-associated protein Snapin; SNAP-associated proteinSNARE associated protein snapin; Snapin; Synaptosomal-associated protein 25-binding protein