• Species Reactivity

  Human

• Specificity

  Detects human Collybistin/ARHGEF9 in direct ELISAs.

• Source

  Monoclonal Mouse IgG    _2B Clone # 809309

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  E. coli-derived recombinant human Collybistin/ARHGEF9    
  Asn390-Lys516    
  Accession # O43307

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Western Blot

  2 µg/mL

  See below

• 
  

• Immunohistochemistry

  8-25 µg/mL

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Sterile PBS to a final concentration of 0.5 mg/mL.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Collybistin/ARHGEF9

Collybistin, also known as ARHGEF9, is an approximately 60 kDa cytoplasmic guanine nucleotide exchange factor. It is expressed in the brain and is upregulated at the onset of neuronal differentiation. Collybistin associates with Neuroligin-2, Gephyrin, and GABA receptor subunits to form a complex which is required for GABAergic synaptogenesis as well as hippocampal synaptic plasticity. Mutations in Collybistin are associated with a variety of neurological disorders including epilepsy, anxiety, aggression, mental retardation, and hyperplexia. Human Collybistin contains an SH3 domain (aa 8-67), a DH domain (aa 103-287), and a Pleckstrin homology domain (aa 318-426). Within aa 390-516, human Collybistin shares 97% and 57% aa sequence identity with mouse and rat Collybistin, respectively.

• Long Name:

  Cdc42 Guanine Nucleotide Exchange Factor (GEF) 9

• Entrez Gene IDs:

  23229 (Human); 236915 (Mouse); 66013 (Rat)

• Alternate Names:

  ARHGEF9; Cdc42 guanine nucleotide exchange factor (GEF) 9; Collybistin; EIEE8; hPEM-2 collybistin; KIAA0424; KIAA0424HPEM-2; PEM-2 homolog; PEM2; PEM-2; Rac/Cdc42 guanine nucleotide exchange factor 9; rho guanine nucleotide exchange factor 9