• Species Reactivity

  Human, Mouse

• Specificity

  Detects human Preso/FRMPD4 in direct ELISAs and human and mouse Preso/FRMPD4 in Western blots.

• Source

  Monoclonal Mouse IgG    _2B Clone # 809408

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  E. coli-derived recombinant human Preso/FRMPD4    
  Asn890-Ser1022    
  Accession # Q14CM0

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Western Blot

  2 µg/mL

  See below

• 
  

• Simple Western

  20 µg/mL

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Sterile PBS to a final concentration of 0.5 mg/mL.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Preso/FRMPD4

Preso, also known as FRMPD4 and PDZD10, is a scaffolding protein that is widely expressed in the brain as 140 kDa and 170 kDa species. It contains one WW domain (aa 33-66) one PDZ domain (aa 78-155), and one FERM domain (aa 204-519). The 140 kDa species is enriched in post-synaptic densities where it interacts with PSD-95, SAP97, SAP102, and F-actin. Preso promotes dendritic spine extension and maintenance as well as excitatory synaptic transmission. Within aa 890-1022, human Preso shares approximately 94% aa sequence identity with mouse and rat Preso.

• Long Name:

  FERM and PDZ Domain Containing 4

• Entrez Gene IDs:

  9758 (Human); 333605 (Mouse); 302656 (Rat)

• Alternate Names:

  FERM and PDZ domain containing 4; FERM and PDZ domain-containing protein 4; FRMPD4; MGC142260; MGC142262; PDZ domain containing 10; PDZD10; PDZK10; Preso