• Species Reactivity

  Human

• Specificity

  Detects human Tissue alpha ‑L‑Fucosidase/FUCA1 in direct ELISAs and Western blots.

• Source

  Monoclonal Mouse IgG    ₁ Clone # 810607

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant human Tissue alpha ‑L‑Fucosidase/FUCA1    
  Gln32-Lys466    
  Accession # P04066

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Western Blot

  0.2 µg/mL

  See below

• 
  

• Simple Western

  2 µg/mL

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Sterile PBS to a final concentration of 0.5 mg/mL.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Tissue alpha-L-Fucosidase/FUCA1

Fucosylated glycoconjugates play numerous roles in biological processes, such as development, apoptosis and the immune responses, and are involved in the pathology of inflammation, cancer and cystic fibrosis (1, 2, 3, 4). Tissue alpha ‑L‑Fucosidase, also known as FUCA1, is a lysosomal enzyme responsible for hydrolyzing alpha ‑L‑fucose moieties from glycolipids and oligosaccharides (5). The mature enzyme in the liver has a molecular weight of 200 kDa and exists in the native state as a tetramer (6). Defects in FUCA1 are the cause of fucosidosis (7, 8, 9), an autosomal recessive lysosomal storage disease characterized by the accumulation of fucose-containing glycolipids and glycoproteins in various tissues. Serum alpha ‑L‑fucosidase has been identified as a useful marker for oral cancer (10).

• References:

1. Hiraishi, K. et al. (1993) Glycobiology 3:381.

2. Solter, D. and Knowles, B.B. (1978) Proc. Natl. Acad. Sci. USA 75:5565.

3. Becker, D.J. and Lowe, J.B. (2003) Glycobiology 13:41R.

4. Liu, T.W. et al. (2009) Proc. Natl. Acad. Sci. USA 106:14581.

5. Fukushima, H. et al. (1985) Proc. Natl. Acad. Sci. USA 82:1262.

6. Alhadeff, J.A. et al. (1975) J. Biol. Chem. 250:7106.

7. O’Brien, J.S. et al. (1987) Enzyme 38:45.

8. Durand, P. et al. (1969) J. Pediatr. 75:665.

9. Johnson, K. and Dawson, G. (1985) Biochem. Biophys. Res. Commun. 133:90.

10. Shah, M. et al. (2008) Cancer 113:336.

• Entrez Gene IDs:

  2517 (Human); 71665 (Mouse); 24375 (Rat)

• Alternate Names:

  Alpha-L-fucosidase 1; Alpha-L-fucosidase I; Alpha-L-fucoside fucohydrolase 1; EC 3.2.1; EC 3.2.1.51; FUCA; FUCA1; fucosidase, alpha-L- 1, tissue; Tissue alphaLFucosidase; Tissue alpha-L-Fucosidase