• Species Reactivity

  Human

• Specificity

  Detects human Serpin E1 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human Serpin A1, A3, A4, A8, C1, F1, F2, I1, I2, recombinant mouse Serpin D1 or E2 is observed.

• Source

  Monoclonal Mouse IgG    ₁ Clone # 242816

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  S. frugiperda insect ovarian cell line     Sf 21-derived recombinant human Serpin E1/PAI-1    
  Met1-Pro402    
  Accession # P05121

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Western Blot

  1 µg/mL

  See below

• 
  

• Simple Western

  2.5 µg/mL

  See below

• 
  

• Human Serpin E1/PAI-1 Sandwich Immunoassay

  Reagent    

• ELISA Capture (Matched Antibody Pair)

  2-8 µg/mL 

  Human Serpin E1/PAI‑1 Antibody (Catalog # )

• ELISA Detection (Matched Antibody Pair)

  0.1-0.4 µg/mL 

  Human Serpin E1/PAI‑1 Biotinylated Antibody (Catalog # )

• ELISA Standard

   

  Recombinant Human Serpin E1/PAI-1 Protein, CF (Catalog # )

• 
  

• Neutralization

  Measured by its ability to neutralize Recombinant Human Serpin E1/PAI-1 (0.25 µg/mL, Catalog # ) inhibition of Recombinant Human u‑Plasminogen Activator (uPA)/Urokinase (0.1 µg/mL, Catalog # ) cleavage of the fluorogenic peptide substrate Z-GGR-AMC (100 µM). The Neutralization Dose (ND     ₅₀) is typically 0.3 µg/mL.

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Serpin E1/PAI-1

As a member of the Serpin superfamily of serine protease inhibitors, Serpin E1/PAI-1 is the principal inhibitor of urokinase-type plasminogen activator (uPA) and tissue-type plasminogen activator (tPA) (1, 2). As important regulators of extracellular matrix remodeling, uPA and PAI-1 play a major role in many processes such as angiogenesis, tumor invasion and obesity (2‑4). For example, uPA and PAI-1 are the only tumor prognostic factors validated at the highest level of evidence with regard to their clinical utility in breast cancer (5). The human PAI-1 is initially synthesized as 402 amino acid precursor with a N-terminal signal peptide (6, 7). PAI-1 may exist in one of two possible conformations, designated as active or latent (8).

• References:

1. Silverman, G.A. et al. (2001) J. Biol. Chem. 276:33293.

2. Stefansson, S. et al. (2003) Curr. Pharm. Des. 9:1545.

3. Duffy, M.J. (2002) Clin. Chem. 48:1194.

4. Juhan-Vague, I. et al. (2003) J. Thromb. Haemost. 1:1575.

5. Harbeck, N. et al. (2002) Clin. Breast Cancer 3:196.

6. Pannekoek, H. et al. (1986) EMBO J. 5:2539.

7. Ginsburg, D. et al. (1986) J. Clin. Invest. 78:1673.

8. Wang, Z. et al. (1996) Biochemistry 35:16443.

9. Stromqvist, M. et al. (1994) Protein Expr. Purif. 5:309.  

• Long Name:

  Plasminogen Activator Inhibitor

• Entrez Gene IDs:

  5054 (Human); 18787 (Mouse)

• Alternate Names:

  Endothelial plasminogen activator inhibitor; Nexin; PAI1; PAI-1; PAI1PAI-1; PAISerpin E1; PLANH1; PLANH1plasminogen activator inhibitor 1; serine (or cysteine) proteinase inhibitor, clade E (nexin, plasminogenactivator inhibitor type 1), member 1; Serpin E1; serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitortype 1), member 1