• Species Reactivity

  Human

• Specificity

  Detects human Carbonic Anhydrase VI in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human Carbonic Anhydrase 1, 2, 3, 4, 5A, 5B, 7, 8, or 9 is observed.

• Source

  Monoclonal Mouse IgG2b Clone # 401809

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant human Carbonic Anhydrase VI    
  Gln18-Asn308    
  Accession # EAW71606

• Formulation

  Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

• Label

  Alexa Fluor 750

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Intracellular Staining by Flow Cytometry

  0.25-1 µg/10    ⁶ cells

  OVCAR‑3 human ovarian carcinoma cell line fixed with paraformaldehyde and permeabilized with saponin

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Carbonic Anhydrase VI

Carbonic Anhydrase catalyzes the reversible reaction of CO₂ + H₂O = HCO₃^- + H⁺, which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption (1). Topics in a CA meeting (6^th International Conference on the CAs, June 20‑25, 2003, Slovakia) ranged from the use of CAs as markers for tumor and hypoxia in the clinic, as a nutritional supplement in milk, and as a tool for CO₂ removal and mosquito control in industry. Carbonic Anhydrase VI, also known as gustin and salivary Carbonic Anhydrase, is a zinc‑metalloprotein that constitutes about 3% of human parotid saliva protein (2, 3). It was decreased in patients with loss of taste and pathological changes in taste buds (4). It is also an elementary component of milk. It plays an important role in normal growth and development of the infant alimentary tract (5).

• References:

1. Hewett-Emmett, D. and R.E. Tashian (1996) Mol. Phylogenet. Evol. 5:50.

2. Murakami, H. and Sly, W. S. (1987) J. Biol. Chem. 262:1382.

3. Thatcher, B. J. et al. (1998) Biochem. Biophys. Res. Commun. 250:635.

4. Hankin, R. I. et al. (1999) Am. J. Med. Sci. 318:380.

5. Karhumaa, P. et al. (2001) Proc. Natl. Acad. Sci. USA. 98:11604.

• Entrez Gene IDs:

  765 (Human); 298657 (Rat)

• Alternate Names:

  CA6; Carbonate dehydratase VI; carbonic anhydrase 6; Carbonic Anhydrase VI; carbonic anhydrase VIGUSTIN; CA-VI; EC 4.2.1.1; Gustin; MGC21256; Salivary carbonic anhydrase; Secreted carbonic anhydrase