• Species Reactivity

  Human, Mouse, Rat

• Specificity

  Detects human MIOS in direct ELISAs and Western blots.

• Source

  Monoclonal Mouse IgG    ₁ Clone # 955413

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  Human MIOS synthetic peptide    
  Accession # Q9NXC5

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Western Blot

  2 µg/mL

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MIOS

MIOS is a member of the octameric GATOR (GTPase-activating protein (GAP) activity toward RAGs) complex.  MIOS and fellow GATOR2 subcomplex members WDR24, WDR59, SEH1L and SEC13 inhibit GATOR1 subcomplex members DEPDC5, NPRL2 and NPRL3.  This inhibition by GATOR2 positively regulates mTORC1 signaling, an activity modulated by energy levels, growth factors, and amino acids.  Human MIOS is 875 amino acids (aa) in length, and contains six N-terminal WD40 repeats and a C-terminal zinc finger-like domain.  Over aa 620-875, human MIOS shares 98% identity with mouse and rat MIOS.

• Long Name:

  Missing Oocyte, Meiosis Regulator

• Entrez Gene IDs:

  54468 (Human); 252875 (Mouse); 362324 (Rat)

• Alternate Names:

  FLJ20323; MIO; MIOS; missing oocyte, meiosis regulator, homolog (Drosophila); WD repeat-containing protein mio