详细说明
Species Reactivity
Human
Specificity
Detects human ORM-2 in direct ELISAs.
Source
Monoclonal Mouse IgG 1 Clone # 959445
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human ORM-2
Met1-Glu201
Accession # P19652Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated
Applications
Recommended
ConcentrationSample
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Immunocytochemistry
5-25 µg/mL
See below
Intracellular Staining by Flow Cytometry
0.25 µg/10 6 cells
See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. are available in the Technical Information section on our website.
Data Examples
Immunocytochemistry | ORM2 in BG01V Human Embryonic Stem Cells. ORM2 was detected in immersion fixed BG01V human embryonic stem cells differentiated into hepatocytes using Mouse Anti-Human ORM2 Monoclonal Antibody (Catalog # MAB9306) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # ) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for . |
Intracellular Staining by Flow Cytometry | Detection of ORM2 in HepG2 Human Cell Line by Flow Cytometry. HepG2 human hepatocellular carcinoma cell line was stained with Mouse Anti-Human ORM2 Monoclonal Antibody (Catalog # MAB9306, filled histogram) or isotype control antibody (Catalog # , open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # ). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # ) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # ). View our protocol for . |
Immunocytochemistry | ORM2 in HepG2 Human Cell Line. ORM2 was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Mouse Anti-Human ORM2 Monoclonal Antibody (Catalog # MAB9306) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # ) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for . |
Preparation and Storage
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: ORM2
Orosomucoid 2 (ORM2) belongs to the family of lipocalin cytosolic fatty-acid binding proteins. ORM2 is a key acute phase plasma protein. Because of its increase due to acute inflammation, this protein is classified as an acute-phase reactant. ORM2 may play a role in immunosuppression.
Long Name:
Orosomucoid 2
Entrez Gene IDs:
5005 (Human)
Alternate Names:
AGP 2; AGP2; AGP2alpha-1-acid glycoprotein 2; AGP-B; AGP-B'; alpha-1-acid glycoprotein, type 2; OMD 2; ORM2; orosomucoid 2; orosomucoid-2
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