• Species Reactivity

  Human

• Specificity

  Detects human FYB/ADAP/SLAP130 in direct ELISAs.

• Source

  Monoclonal Mouse IgG2b Clone # 460107

• Immunogen

  E. coli-derived recombinant human FYB/ADAP/SLAP130    
  Ala2-Gly274    
  Accession # O15117

• Formulation

  Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

• Label

  Alexa Fluor 750

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Intracellular Staining by Flow Cytometry

  0.25-1 µg/10    ⁶ cells

  Jurkat human acute T cell leukemia cell line fixed with paraformaldehyde and permeabilized with methanol

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: FYB/ADAP/SLAP130

Human SH2 domain containing leukocyte protein-76-Associated Phosphoprotein of 130 kDa (SLAP130), also known as Fyb-120 and ADAP, is an 86 kDa, 783 amino acid, cytoplasmic protein that runs anomalously as a 120‑130 kDa protein in SDS-PAGE. Human SLAP130 is a hematopoietic-specific adaptor that associates with other cytoplasmic proteins such as SH2-containing Leukocyte Phosphoprotein of 76 kDa (SLP-76). Adaptors integrate signals from surface receptors and are essential components of many immune cell-signaling cascades. There is at least one known alternatively-spliced SLAP130 isoform which contains an additional 46 aa. Over the region used as immunogen, SLAP130 is 66% and 76% identical to the corresponding canine and mouse proteins, respectively.

• Long Name:

  FYN Binding Protein (FYB-120/130)

• Entrez Gene IDs:

  2533 (Human); 23880 (Mouse); 499537 (Rat)

• Alternate Names:

  ADAP; Adhesion and degranulation promoting adaptor protein; adhesion and degranulation-promoting adaptor protein; FYB; FYB-120/130SLAP-130FYN-binding protein (FYB-120/130); FYN binding protein; FYN-binding protein; FYN-T-binding protein; p120/p130; PRO0823; SLAP130; SLP-76-associated phosphoprotein