• Species Reactivity

  Mouse

• Specificity

  Detects mouse CD14 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 10% cross-reactivity with recombinant human CD14 is observed.

• Source

  Monoclonal Rat IgG2a Clone # 159010

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant mouse CD14    
  Ala18-Pro345 (predicted)    
  Accession # P10810

• Formulation

  Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

• Label

  Alexa Fluor 750

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Flow Cytometry

  0.25-1 µg/10    ⁶ cells

  Mouse whole blood

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CD14

CD14 is a 55 kDa cell surface glycoprotein that is preferentially expressed on monocytes/macrophages. The mouse CD14 cDNA encodes a 366 amino acid (aa) residue precursor protein with a 15 aa signal peptide and a C-terminal hydrophobic region characteristic for glycosylphosphatidyinositol (GPI)-anchored proteins. Mouse CD14 has five potential N-linked glycosylation sites and also bears O-linked carbohydrates. The amino acid sequence of mouse CD14 is approximately 65% and 82% identical to the human and rat proteins, respectively. CD14 is a pattern recognition receptor that binds lipopolysaccharides (LPS) and a variety of ligands derived from different microbial sources. The binding of CD14 with LPS is catalyzed by LPS-binding protein (LBP). The toll-like-receptors have also been implicated in the transduction of CD14-LPS signals. Similar to other GPI-anchored proteins, soluble CD14 can be released from the cell surface by phosphatidyinositol-specific phospholipase C. Soluble CD14 has been detected in serum and body fluids. High concentrations of soluble CD14 have been shown to inhibit LPS-mediated responses. However, soluble CD14 can also potentiate LPS response in cells that do not express cell surface CD14.

• References:

1. Wright, S.D. et al. (1990) Science 249:1431.

2. Pugin, J. et al. (1993) Proc. Natl. Acad. Sci. USA 90:2744.

3. Beutler, B. (2000) Current Opinion in Immunology 12:20.

4. Stelter, F. (2000) Chem. Immunol. 74:25.

• Entrez Gene IDs:

  929 (Human); 12475 (Mouse); 60350 (Rat); 100037938 (Porcine)

• Alternate Names:

  CD14 antigen; CD14 molecule; CD14; monocyte differentiation antigen CD14; Myeloid cell-specific leucine-rich glycoprotein