• Species Reactivity

  Human

• Specificity

  Detects human TDRKH in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human TDRD1 is observed.

• Source

  Monoclonal Mouse IgG    _2A Clone # 686213

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  E. coli-derived recombinant human TDRKH    
  His206-Leu561    
  Accession # Q9Y2W6

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Immunohistochemistry

  8-25 µg/mL

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Sterile PBS to a final concentration of 0.5 mg/mL.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TDRKH

TDRKH (Tudor domain and KH domain containing protein; also TDRD2) is a 70 kDa member of the Tudor family of proteins.  It is expressed in developing spermatogonia and meiotic primary spermatocytes, but not embryonic stem cells.  TDRKH forms part of an RNA-silencing complex that safeguards the germ cell genome.  Through its 60 amino acid (aa) Tudor domain, it binds methylated PIWI (P-element-induced wimpy testis) protein and serves to position PIWI for noncoding RNA binding.  Human TDRKH is 606 aa in length.  It contains two RNA recognition KH domains (aa 52-115 and 124-190), and one methyl-binding Tudor domain (aa 353-412).  There are four potential splice forms.  Three possess a shared deletion of aa 562-606, with two of these also showing a unique deletion of aa 108-152 and 76-79, respectively; a fourth shows a two aa substitution for aa 559-606.  Over aa 206-561, human TDRKH shares 87% aa identity with mouse TDRKH.          

• Long Name:

  Tudor and KH Domain Containing

• Entrez Gene IDs:

  11022 (Human); 72634 (Mouse); 310652 (Rat)

• Alternate Names:

  TDRD2; TDRKH; tudor and KH domain containing; tudor and KH domain-containing protein