详细说明
Species Reactivity
Human
Specificity
Detects human ApoA1 in direct ELISAs and Western blots.
Source
Recombinant Monoclonal Rabbit IgG Clone # 2083A
Purification
Protein A or G purified from cell culture supernatant
Immunogen
E. coli-derived recombinant human ApoA1
Asp25-Gln267
Accession # P02647Formulation
Supplied as a solution in PBS containing BSA, Glycerol and Sodium Azide. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated
Applications
Recommended
ConcentrationSample
Western Blot
0.2 µg/mL
See below
Flow Cytometry
0.25 µg/10 6 cells
See below
Immunohistochemistry
1-25 µg/mL
See below
Immunocytochemistry
1-25 µg/mL
See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. are available in the Technical Information section on our website.
Data Examples
Western Blot | Detection of Human Apolipoprotein A‑I/ ApoA1 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and Hep3B human hepatocellular carcinoma cell line. PVDF membrane was probed with 0.2 µg/mL of Rabbit Anti-Human Apolipoprotein A‑I/ApoA1 Monoclonal Antibody (Catalog # MAB36641) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # ). A specific band was detected for Apolipoprotein A‑I/ApoA1 at approximately 25-30 kDa (as indicated). This experiment was conducted under reducing conditions and using . |
Flow Cytometry | Detection of Apolipoprotein A‑I/ApoA1 in HepG2 Human Cell Line by Flow Cytometry. HepG2 human hepatocellular carcinoma cell line was stained with Rabbit Anti-Human Apolipoprotein A‑I/ApoA1 Monoclonal Antibody (Catalog # MAB36641, filled histogram) or isotype control antibody (Catalog # , open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # ). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # ) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # ). View our protocol for . |
Immunocytochemistry | Apolipoprotein A‑I/ApoA1 in Human Hepatocytes. Apolipoprotein A‑I/ApoA1 was detected in immersion fixed human hepatocytes using Rabbit Anti-Human Apolipoprotein A‑I/ApoA1 Monoclonal Antibody (Catalog # MAB36641) at 1 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # ) and counterstained with DAPI (blue). Specific staining was localized to cell secretion. View our protocol for . |
Immunocytochemistry | Apolipoprotein A‑I/ApoA1 in HepG2 Human Cell Line. Apolipoprotein A‑I/ApoA1 was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Rabbit Anti-Human Apolipoprotein A‑I/ApoA1 Monoclonal Antibody (Catalog # MAB36641) at 1 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # ) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for . |
Immunohistochemistry | Apolipoprotein A‑I/ApoA1 in Human Liver Cancer Tissue. Apolipoprotein A‑I/ApoA1 was detected in immersion fixed paraffin-embedded sections of human liver cancer tissue using Rabbit Anti-Human Apolipoprotein A‑I/ApoA1 Monoclonal Antibody (Catalog # MAB36641) at 1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # ). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in cancer cells. View our protocol for . |
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C, as supplied.
1 month, 2 to 8 °C under sterile conditions after opening.
6 months, -20 to -70 °C under sterile conditions after opening.
Background: Apolipoprotein A-I/ApoA1
ApoA1 is a major protein component of serum high-density lipoprotein (HDL) and is produced by the liver and small intestine. It is involved in reverse cholesterol transport from tissues to the liver. ApoA1 is synthesized as a 267 amino acid (aa) precursor from which a signal peptide and short propeptide are removed. Mature human ApoA1 shares 65% and 61% aa sequence identity with mouse and rat ApoA1, respectively.
Entrez Gene IDs:
335 (Human); 11806 (Mouse); 25081 (Rat)
Alternate Names:
Alp-1; APOA1; Apo-AI; apoA-I; Apolipoprotein A1; Apolipoprotein AI; Apolipoprotein A-I; Brp-14; Ltw-1; Lvtw-1; MGC117399; Sep-1; Sep-2
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