• Species Reactivity

  Human, Mouse, Rat

• Specificity

  Detects human GRK2 in direct ELISAs and Western blots.    
   

• Source

  Recombinant Monoclonal Rabbit IgG Clone # 2089B

• Purification

  Protein A or G purified from cell culture supernatant

• Immunogen

  E.coli-derived recombinant human GRK2    
  Pro468-Leu689    
  Accession # P25098

• Formulation

  Supplied as a solution in PBS containing BSA, Glycerol and Sodium Azide. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Western Blot

  0.1 µg/mL

  See below

• 
  

• Simple Western

  1 µg/mL

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C, as supplied.

• 1 month, 2 to 8 °C under sterile conditions after opening.

• 6 months, -20 to -70 °C under sterile conditions after opening.

Background: GRK2

G protein-coupled receptor kinases (GRKs) are important modulators of G protein-coupled receptor (GPCR) signaling. Receptor phosphorylation by specific GRKs plays a key role in triggering rapid desensitization. The GRK family consists of 7 isoforms that share a central catalytic domain with homology to other serine/threonine kinases. The catalytic domain is flanked by an amino-terminal RGS domain of 183‑188 amino acids and a carboxyl-terminus of variable length. GRK2 is a member of the beta ARK subfamily, and was originally termed beta ‑adrenergic receptor kinase 1 ( beta ARK1).

• Long Name:

  G protein-coupled Receptor Kinase 2

• Entrez Gene IDs:

  156 (Human); 110355 (Mouse); 25238 (Rat)

• Alternate Names:

  ADRBK1; adrenergic, beta, receptor kinase 1; BARK; BARK1; BARK1beta-ARK-1; Beta-ARK1; Beta-ARK-1; EC 2.7.11; EC 2.7.11.15; FLJ16718; G-protein coupled receptor kinase 2; GRK2; GRK2beta-adrenergic receptor kinase 1