• Species Reactivity

  Human, Mouse, Rat

• Specificity

  Detects human STK3 in direct ELISAs and Western blots.    
   

• Source

  Recombinant Monoclonal Rabbit IgG Clone # 2072A

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  E. coli-derived recombinant human STK3    
  Ala339-Asp456    
  Accession # Q13188

• Formulation

  Supplied as a solution in PBS containing BSA, Glycerol and Sodium Azide. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Western Blot

  0.1 µg/mL

  See below

• 
  

• Simple Western

  1 µg/mL

  See below

• 
  

• Immunohistochemistry

  0.3-25 µg/mL

  See below

• 
  

• Immunocytochemistry

  3-25 µg/mL

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C, as supplied.

• 1 month, 2 to 8 °C under sterile conditions after opening.

• 6 months, -20 to -70 °C under sterile conditions after opening.

Background: MST2/STK3

MST2 (mammalian sterile twenty-like 2; also known as STK3 and Krs-1) is a 58 kDa member of the GCKII group of the STE20 subfamily of the STE Ser/Thr kinase protein family. In human, MST2 is 491 amino acids (aa) in length. It contains one kinase domain (aa 27‑278), two NESs (aa 361‑371 and 438‑447) and coiled-coil domains (aa 287‑328 and 442‑475), and one NLS (aa 473‑487). A caspase-cleavage site exists between Asp322‑Ser323. MST2 is normally inactive in the cytoplasm bound to Raf-1. Cell activation induces Raf-1:MST2 dissociation, MST2 cleavage, and N-terminal translocation to the nucleus. Human MST2 is 99% and 96% aa identical to canine and mouse MST2, respectively.

• Entrez Gene IDs:

  6788 (Human); 56274 (Mouse)

• Alternate Names:

  EC 2.7.11; FLJ90748; KRS1; Mammalian STE20-like protein kinase 2; Mess1; MST2; MST-2; MST2EC 2.7.11.1; MST3; serine/threonine kinase 3 (STE20 homolog, yeast); serine/threonine kinase 3 (Ste20, yeast homolog); serine/threonine kinase 3; serine/threonine-protein kinase 3; Serine/threonine-protein kinase Krs-1; STE20-like kinase MST2; STK3