• Species Reactivity

  Human

• Specificity

  Detects human GABARAP in direct ELISAs.

• Source

  Monoclonal Rat IgG    _2A Clone # 853641

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  E. coli-derived recombinant human GABARAP    
  Accession # O95166

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

• Label

  Unconjugated

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Western Blot

  2 µg/mL

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GABARAP

gamma -Aminobutyric Acid Receptor-associated Protein (GABARAP), also known as Apg8p1, is 117 amino acid (aa) protein with a predicted molecular weight of 14 kDa. It is a member of the GABARAP subfamily of the Autophagy-related 8 (Atg8) family of proteins (1). The GABARAP subfamily also includes GABARAPL1. GABARAP/Apg8p1 has 100% aa sequence identity with its mouse and rat orthologs and is orthologous to yeast Atg8. Atg8 family members show structural similarity with Ubiquitin, but lack aa sequence similarity. GABARAP/Apg8p1 was first described for its putative involvement GABA A Receptor trafficking (2,3). However, it is best known for its role in autophagy (1,4). GABARAP/Apg8p1 covalently attaches to phosphatidylethanolamine (PE) in the phagophore (autophagosome precursor) membrane using a Ubiquitin-like conjugation system that includes Ubiquitin-activating (E1)-, Ubiquitin-conjugating (E2)-, and Ubiquitin Ligase (E3)-like enzymes. Here it is involved in the later stages of autophagosome maturation (4,5). It may also be involved in cargo recruitment to autophagosomes (1).

• References:

1. Shpilka, T. et al. (2011) Genome Biol. 12:226.

2. Wang, H. et al. (1999) Nature 397:69.

3. Leil, T.A. et al. (2004) J. Neurosci. 24:11429.

4. Weidberg, H. et al. (2010) EMBO J. 29:1792.

5. Weidberg, H. et al. (2011) Ann. Rev. Biochem. 80:125.

• Long Name:

  gamma-Aminobutyric Acid Type A Receptor Associated Protein

• Entrez Gene IDs:

  11337 (Human); 56486 (Mouse); 58974 (Rat)

• Alternate Names:

  Apg8p1; ATG8A; FLC3B; FLJ25768; GABA(A) receptor-associated proteinMGC120154; GABARAP; gamma-aminobutyric acid receptor-associated protein; MM46; MM46MGC120155