• Species Reactivity

  Human, Mouse

• Specificity

  Detects human YY1 in ELISAs. In direct ELISAs, no cross-reactivity with recombinant human Rex-1/ZFP42 is observed.

• Source

  Monoclonal Mouse IgG    _2B Clone # 854847

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  E. coli-derived recombinant human YY1    
  His75-Gln275    
  Accession # P25490

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Western Blot

  0.5 µg/mL

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Sterile PBS to a final concentration of 0.5 mg/mL.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: YY1

YY1 (Yin Yang 1) is a multifunctional transcription factor belonging to the GLI-Kruppel class of zing finger proteins. YY1 is essential for embryogenesis, apoptosis, differentiation, cell growth and proliferation. YY1 binds to the DNA consensus site CGCCATNTT. It can function as an initiator of transcription as well as activating or repressing transcription through protein complexes (e.g. histone deacetylases and histone acetyltransferases).

• Long Name:

  Yin-Yang-1

• Entrez Gene IDs:

  7528 (Human); 22632 (Mouse); 24919 (Rat)

• Alternate Names:

  Delta transcription factor; DELTA; INO80SYY-1; NF-E1; NF-E1INO80 complex subunit S; transcriptional repressor protein YY1; UCRBP; Yin and Yang 1 protein; Yin and yang 1; YIN-YANG-1; YY1 transcription factor; YY1