• Species Reactivity

  Mouse

• Specificity

  Detects mouse Klre-1 in ELISAs.

• Source

  Monoclonal Rat IgG    _2B Clone # 854929

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  Chinese hamster ovary cell line, CHO derived recombinant mouse Klre-1    
  Lys94-Lys226    
  Accession # NP_705818

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Flow Cytometry

  0.25 µg/10    ⁶ cells

  See below

• 
  

• CyTOF-ready

  Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Sterile PBS to a final concentration of 0.5 mg/mL.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Klre-1

Killer cell lectin-like receptor family E member 1 (Klre-1), also called NKG2I (NKG2 family member I), is a 24-28 kDa member of the NKG2/KLR family of proteins. It is expressed on mouse NK and NKT cells, and appears to serve as one component of two novel heterodimeric cell surface receptors. When complexed to KLRI1, Klre-1 inhibits NK cell cytotoxic activity. When complexed to KLRI2, Klre-1 activates NK cells, inducing IFN-gamma production and the activation of a cytolytic program. Although Klre-1 preferentially associates noncovalently with NLRI1 and I2, it apparently will form disulfide-linked homodimers in the absence of its heterodimer partners. Mouse Klre-1 is a 226 amino acid (aa) type II transmembrane protein. It contains an N-terminal cytoplasmic segment (aa 1-68) plus a 133 aa extracellular region (aa 94-226) that possesses one C-type lectin domain (aa 110-226). Over aa 94-226, mouse Klre-1 shares 82% aa identity with rat Klre-1. There does not appear to be a human structural ortholog to mouse Klre-1.

• Long Name:

  Killer Cell Lectin-like Receptor Family E Member 1

• Entrez Gene IDs:

  243655 (Mouse)

• Alternate Names:

  Klre1; Klre-1; NKG2I