• Species Reactivity

  Human

• Specificity

  Detects human ELA2 in direct ELISAs and Western blots.    
   

• Source

  Monoclonal Mouse IgG    ₁ Clone # 950317

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  Chinese hamster ovary cell line CHO-derived recombinant human ELA2    
  Met1-Asn252    
  Accession # P08246

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Western Blot

  0.1 µg/mL

  See below

• 
  

• Immunohistochemistry

  3-25 µg/mL

  See below

• 
  

• CyTOF-ready

  Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

• 
  

• Immunocytochemistry

  5-25 µg/mL

  See below

• 
  

• Intracellular Staining by Flow Cytometry

  0.25 µg/10    ⁶ cells

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Neutrophil Elastase/ELA2

Neutrophil Elastase (ELA2, ELANE), also known as HNE, is a chymotrypsin family serine protease that plays a key role in pathogen clearance (1-3). It is expressed by promyelocytes and stored in the intracellular azurophilic granules of polymorphonuclear leukocytes (PMN) (4). These granules fuse with phagosomes, enabling Neutrophil Elastase to participate in the digestion and killing of endocytosed microbes. The enzyme is released by activated neutrophils at sites of inflammation, and it can remain associated with the cell surface or function as a component of neutrophil extracellular nets (NETs) which trap and kill microbial pathogens (5, 6). It also can degrade multiple extracellular matrix proteins including Elastin and Fibronectin (5). In the lung, this activity contributes to pathology in emphysema, cystic fibrosis, and adult respiratory distress syndrome (ARDS) (1). Neutrophil Elastase can be inhibited by Serpin A1/alpha 1-Antitrypsin, SLPI, Serpin B1, and  
Trappin-2/Elafin (7-11). Its activity in the lung is increased by exposure to tobacco smoke which inactivates Serpin A1 through methionine oxidation (12). Mature human Neutrophil Elastase shares 73% amino acid sequence identity with mouse and rat Neutrophil Elastase (13, 14). Multiple mutations in the human ELANE gene are causative of severe congenital and cyclic neutropenias (15).

• References:

1. Korkmaz, B. et al. (2010) Pharmacol. Rev. 62:726.

2. Stein, R.L. et al. (1987) Biochemistry 26:1301.

3. Bachovchin, W.W. (1986) Biochemistry 25:7751.

4. Garwicz, D. et al. (2005) Haematologica 90:38.

5. Owen, C.A. et al. (1995) J. Cell Biol. 131:775.

6. Stephan, A. and M. Fabri (2015) Exp. Dermatol. 24:161.

7. Carrell, R.W. et al. (1982) Nature 298:329.

8. Rice, W.G. and S.J. Weiss (1990) Science 249:178.

9. Thompson, R.C. et al. (1986) Proc. Natl. Acad. Sci. USA 83:6692.

10. Cooley, J. et al. (2001) Biochemistry 40:15762.

11. Wiedow, O. et al. (1990) J. Biol. Chem. 265:14791.

12. Taggart, C. et al. (2000) J. Biol. Chem. 275:27258.

13. Sinha, S. et al. (1987) Proc. Natl. Acad. Sci. USA 84:2228.

14. Okano, K. et al. (1987) J. Biochem. 102:13.

15. Makaryan, V. et al. (2015) Curr. Opin. Hematol. 22:3.

• Entrez Gene IDs:

  1991 (Human); 50701 (Mouse)

• Alternate Names:

  Bone marrow serine protease; EC 3.4.21; EC 3.4.21.37; ELA2; ELA2granulocyte-derived elastase; ELANE; elastase 2, neutrophil; elastase, neutrophil expressed; Elastase-2; GE; HLEelastase-2; HNE; Human leukocyte elastase; Leukocyte Elastase; Medullasin; NE; Neutrophil Elastase; PMN elastase; PMN-E; polymorphonuclear elastase; SCN1