• Species Reactivity

  Human

• Specificity

  Detects human ATAD2 in direct ELISAs.

• Source

  Monoclonal Mouse IgG    _2B Clone # 952947

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  Human ATAD2 synthetic peptide    
  Accession # Q6PL18

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Immunocytochemistry

  8-25 µg/mL

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ATAD2

ATAD2/ANCCA/PRO2000 is an AAA (ATPases Associated with diverse cellular Activities) protein with two AAA domains and a bromodomain. ATAD2 is a coactivator in the regulatory process of ER alpha induction of target genes in breast cancer. Upon activation by estrogen, ATAD2 associates with ER alpha and ACTR and subsequently promotes expression of ER alpha target genes cyclin D1, c-myc and E2F1 in breast cancer cells.  ATAD2 also binds and hydrolyzes ATP, mediating hypermethylation of protein complexes. ATAD2 is reported to be a prognostic biomarker in endometrial, lung, ovarian and gastric cancers. The function of ATAD2 has also been reported in other carcinomas, including cervical, hepatocellular, colorectal and lung cancers.

• Long Name:

  ATPase family, AAA domain containing 2

• Entrez Gene IDs:

  29028 (Human); 70472 (Mouse); 314993 (Rat)

• Alternate Names:

  AAA nuclear coregulator cancer-associated protein; ANCCA; ANCCAMGC131938; ATAD2; ATPase family AAA domain-containing protein 2; ATPase family, AAA domain containing 2; CT137; DKFZp667N1320; EC 3.6.1.3; MGC142216; MGC29843; MGC5254; PRO2000