• Species Reactivity

  Human

• Specificity

  Stains human TSPAN7 transfectants but not irrelevant transfectants in flow cytometry.

• Source

  Monoclonal Mouse IgG    _2A Clone # 482618

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  HEK293 human embryonic kidney cell line transfected with human TSPAN7    
  Accession # P41732

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Flow Cytometry

  0.25 µg/10    ⁶ cells

  See below

• 
  

• CyTOF-ready

  Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TSPAN7

Tetraspanin-7 (aka TSPAN7,  TALLA-1, TM4SF2 and CD231) is a 249 aminoacids (aa) multi-pass membrane protein.  Tetraspanin proteins regulate morphology, signaling and trafficking processes by interaction and association with proteins in tetraspanin enriched microdomains (TEMs). It has been proposed that tetraspanins interact with integrins to affect cell migration, probably by modulation or compartmentalization of integrin signaling. TSPAN7 has been found to be upregulated in myeloma development and described as a prognostic marker of renal cell carcinoma. Mutations in TSPAN7 are also implicated in some forms of X-linked intellectual disability.

• Long Name:

  Tetraspanin 7

• Entrez Gene IDs:

  7102 (Human); 21912 (Mouse); 363447 (Rat)

• Alternate Names:

  A15; A15CCG-B7; CCG-B7; CD231 antigen; CD231; Cell surface glycoprotein A15; DXS1692E; Membrane component chromosome X surface marker 1; mental retardation, X-linked 58; MRX58; MXS1; MXS1T-cell acute lymphoblastic leukemia associated antigen 1; TALLA-1; T-cell acute lymphoblastic leukemia-associated antigen 1; tetraspanin 7; tetraspanin-7; TM4SF2; TM4SF2b; TM4SF2tetraspanin protein; transmembrane 4 superfamily 2b; Transmembrane 4 superfamily member 2X chromosome, surface marker 1; transmembrane protein A15; TSPAN7; tspan-7