• Species Reactivity

  Mouse

• Specificity

  Detects mouse uPA in direct ELISAs and Western blots.    
   

• Source

  Monoclonal Rat IgG    _2A Clone # 901420

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant mouse uPA    
  Met1-Phe433    
  Accession # P06869

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Western Blot

  1 µg/mL

  See below

• 
  

• Immunohistochemistry

  5-25 µg/mL

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: u-Plasminogen Activator (uPA)/Urokinase

Urokinase-type plasminogen activator (uPA) is a 378 amino acid enzyme which cleaves the zymogen plasminogen to form the active enzyme plasmin. uPa occurs in high and low molecular mass forms each consisting of two chains (A and B).  The high mass form has a long A chain while the low mass form has a short chain AuPA is widely expressed in normal tissues including epithelia, muscle, reproductive organs and brain, and  is highly expressed in kidney, where it localizes to proximal and distal tubules and is secreted into urine. uPA is known to play a role in wound healing and  tissue remodeling and  tumor invasion

• Long Name:

  Urokinase-type Plasminogen Activator

• Entrez Gene IDs:

  5328 (Human); 18792 (Mouse)

• Alternate Names:

  ATF; EC 3.4.21; EC 3.4.21.73; plasminogen activator, urokinase; PLAU; uPA; u-PA; uPlasminogen Activator; u-Plasminogen Activator; urinary; Urokinase; urokinase-type plasminogen activator