• Species Reactivity

  Human

• Specificity

  Detects human TSPAN1 in direct ELISAs.    
   

• Source

  Monoclonal Mouse IgG    ₃ Clone # 819202

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant human TSPAN1    
  Met1-Gln241    
  Accession # O60635

• Formulation

  Lyophilized from a 0.2 μm filtered solution in TBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Flow Cytometry

  0.25 µg/10    ⁶ cells

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TSPAN1

Tetraspanins (TSPAN family) are small integral proteins that contain four transmembrane domains with two different and well characterized extracellular domains. Tetraspanins are found on the plasma membrane and within various intracellular organelles and granules in nearly all cell and tissue types. They interact with one another and with other transmembrane molecules, including integrins, generating a ‘web' of interactions believed to control cell and membrane trafficking and compartmentalization. Tetraspanin-1 (TSPAN1), a 241 amino acid protein, interacts with the thiamine transporter-1 (THTR-1, SLC19A2) in human intestinal epithelial cells, linking TSPAN1 to the regulation of thiamine uptake. TSPAN1 has been found to be expressed in gastric, ovarian, colon, and other carcinomas.

• Long Name:

  Tetraspanin 1

• Entrez Gene IDs:

  10103 (Human); 66805 (Mouse); 298436 (Rat)

• Alternate Names:

  NET1; NET-1; tetraspan 1; Tetraspan NET-1; tetraspanin 1; Tetraspanin TM4-C; tetraspanin-1; TM4C; TM4SF; TSPAN1; TSPAN-1