• Species Reactivity

  Human

• Specificity

  Detects HEK293 human embryonic kidney cell line transfected with human Dopamine D5 R/DRD5 by Flow Cytometry. Does not detect untransfected or irrelevant transfected HEK293 cells.

• Source

  Monoclonal Mouse IgG    _2A Clone # 889022

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  NS0 mouse myeloma cell line transfected with human Dopamine D5 R/DRD5    
  Met1-His477    
  Accession # P21918

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Flow Cytometry

  0.25 µg/10    ⁶ cells

  See below

• 
  

• CyTOF-ready

  Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Dopamine D5 R/DRD5

DRD5, also known as Dopamine D5 Receptor has a higher affinity for neurotransmitter dopamine than DRD1 (1). DRD5 is expressed in neurons in many human brain regions, including cortex regions, hippocampus, choroid plexus, and brainstem (2). Polymorphisms in the DRD5 gene have been associated with Attention Deficit Hyperactivity Disorder (ADHD) (3), schizopherenia (4) and nicotine dependence (5). Dopamine receptors undergo endocytosis upon interaction with receptor agonists and by activation of Protein Kinase C (PKC) (6).

• References:

1. Sunahra, R. et al. (1991) Nature. 350:614.

2. Weinshank, R. et al. (1991) J Biol Chem. 266:22427.

3. Squassina, A. et al.  (2008) Neurosci Lett. Feb 13.

4. Golimbet, V. et al.  (2008) Bull Exp Biol Med. Jan.

5. Wei, J. et al.  (2012) Addict Behav. 37:622.

6. Thompson, D. et al. (2011) Traffic. 12:644.

• Long Name:

  Dopamine D5 Receptor

• Entrez Gene IDs:

  1816 (Human); 13492 (Mouse); 25195 (Rat)

• Alternate Names:

  D(1B) dopamine receptor; D(5) dopamine receptor; D1beta dopamine receptor; DBDR; Dopamine D5 R; Dopamine D5 receptor; Dopamine D5R; dopamine receptor D1B; dopamine receptor D5; DRD1B; DRD1BDBDR; DRD1L2; DRD1L2MGC10601; DRD5