详细说明
Species Reactivity
Human
Specificity
Stains human GPR26 transfectants but not irrelevant transfectants in flow cytometry.
Source
Monoclonal Mouse IgG 2B Clone # 891318
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
NS0 mouse myeloma cell line transfected with human GPR26
Met1-Glu337
Accession # Q8NDV2Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated
Applications
Recommended
ConcentrationSample
Flow Cytometry
0.25 µg/10 6 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Please Note: Optimal dilutions should be determined by each laboratory for each application. are available in the Technical Information section on our website.
Data Examples
Flow Cytometry | Detection of GPR26 in HEK293 Human Cell Line Transfected with Human GPR26 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with human GPR26 and eGFP was stained with either (A) Mouse Anti-Human GPR26 Monoclonal Antibody (Catalog # MAB8449) or (B) Mouse IgG2B Flow Cytometry Isotype Control (Catalog # ) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # ). |
Preparation and Storage
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: GPR26
GPR26 is a brain-specific orphan G Protein-Coupled Receptor (GPCR) of the rhodopsin family. It is frequently reduced or absent in glioma cells and biopsies. Glioblastomas are highly malignant brain tumors. GPR26 has been proposed to be a genetic indicator of primary glioblastoma, suggesting that it could be a suppressor of primary glioblastoma development.
Long Name:
G Protein-coupled Receptor 26
Entrez Gene IDs:
2849 (Human); 233919 (Mouse)
Alternate Names:
DKFZp761H2121; FLJ33774; G protein-coupled receptor 26; GPR26; G-protein coupled receptor 26; MGC138216







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