• Species Reactivity

  Human

• Specificity

  Detects human FSH beta in direct ELISAs and Western blots.    
   

• Source

  Monoclonal Rat IgG    _2A Clone # 961416

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  E.coli-derived recombinant human FSH beta    
  Asn19-Glu129    
  Accession # P01225

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Western Blot

  2 µg/mL

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FSH beta

Follicle-stimulating hormone (FSH; also follitropin) is a 33 kDa heterodimer belonging to the glycoprotein hormones subunit beta family (1). The heterodimer is composed of two glycoproteins linked noncovalently: a common alpha subunit, which is also a component of luteinizing hormone, thyroid stimulating hormone, and chorionic gonadotropin; and a unique beta subunit that confers the protein’s specific biological action and is responsible for the interaction with FSH-receptor. The alpha subunit is 20 kDa and is synthesized as a 116 amino acid (aa) precursor that contains a 24 aa signal sequence and a 92 aa mature chain, which contains two potential sites of N-linked glycosylation (1-2). The beta subunit is 23 kDa and is synthesized as a 129 aa precursor with an 18 aa signal sequence and a 111 aa mature chain, which also contains two potential sites of N-linked glycosylation (1). Human FSH beta shares approximately 90% aa sequence identity with mouse and rat FSH beta. FSH is secreted by gonadotropes of the anterior pituitary gland.  Its release is controlled by pulses of gonadotropin-releasing hormone (GnRH), and those pulses, in turn, are subject to the estrogen feedback from the gonads. FSH regulates the development, growth, pubertal maturation, and reproductive processes of the human body. It promotes follicle maturation and spermatogenesis through interactions with FSH-receptor.

• References:

  
     

1. Van de Wiel, D.F. et al. (1998) J. Mol. Endocrinol. 20:83.

2. Rathnam, P. and B.B. Saxena (1975) J. Biol. Chem. 250:6735.

• Long Name:

  Follicle Stimulating Hormone, beta

• Entrez Gene IDs:

  2488 (Human); 14308 (Mouse); 25447 (Rat)

• Alternate Names:

  follicle stimulating hormone, beta polypeptide; Follicle-stimulating hormone beta subunit; Follitropin beta chain; follitropin subunit beta; Follitropin; follitropin, beta chain; FSH beta; FSHB; FSH-B; FSH-beta