• Species Reactivity

  Human

• Specificity

  Detects human TMIGD2/CD28H in direct ELISAs. Stains human TMIGD2/CD28H transfectants but not irrelevant transfectants in flow cytometry.

• Source

  Monoclonal Mouse IgG    _2B Clone # 953728

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  HEK293 human embryonic kidney cell line-derived human TMIGD2/CD28H    
  Met1-Gly150    
  Accession # Q96BF3

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Flow Cytometry

  0.25 µg/10    ⁶ cells

  See below

• 
  

• Immunohistochemistry

  5-25 µg/mL

  See below

• 
  

• CyTOF-ready

  Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TMIGD2/CD28H

CD28 homolog (CD28H), also called TMIG2 and IGPR-1, is a 55 kDa glycosylated transmembrane protein that shares approximately 10% amino acid (aa) sequence identity with CD28, CTLA-4, ICOS, and PD-1. CD28H is composed of a single extracellular immunoglobulin variable-like domain (IgV) (aa 23-109), a transmembrane domain (aa 151-171), and a long cytoplasmic domain (aa172-282). CD28H is constitutively expressed on naive T and NK cells. Similar to the interaction of B7 with CD28, the interaction of CD28H with B7-H7 activates the Akt-dependent signaling cascade and promotes the proliferation and activation of newly generated peripheral effector and memory T cells (1, 2). CD28H is additionally expressed in the skin and epithelium lining the lung, airway, mammary gland, and gastrointestinal tract (3). It regulates cellular morphology, focal adhesion contact formation, and cell migration (3, 4). CD28H also participates in angiogenesis   in vitro (3). CD28H interacts with multiple cytoskeletal proteins including Actin, Paxillin, SPIN90, CACNB2, and BPAG1. Interactions between the cytoplasmic proline-rich domain of CD28H and SPIN90 modulate the activity of CD28H in both angiogenesis and cell adhesion (3, 5).

• References:

1. Zhu, Y. et al. (2013) Nat. Commun. 4:2043.

2. Parry, R.V. et al. (2003) J.Immunol. 171:166.

3. Rahimi, N. et al. (2012) Mol. Biol. Cell. 23:1646.

4. Huang, C. et al. (2003) Nature 424:219.

5. Kaneko, T. et al. (2008) Front. Biosci. 13:4938.

• Long Name:

  Transmembrane And Immunoglobulin Domain Containing 2

• Entrez Gene IDs:

  126259 (Human)

• Alternate Names:

  CD28H; IGPR1; IGPR-1; MGC23244; TMIGD2; transmembrane and immunoglobulin domain containing 2; transmembrane and immunoglobulin domain-containing protein 2