• Specificity

  Detects N-terminal,C-terminal, and Internal Sequence HA-tagged proteins in Western blot.

• Source

  Monoclonal Mouse IgG1 Clone # 912426

• Immunogen

  HA Tag peptide    
  Accession # ABB51961

• Formulation

  Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

• Label

  Alexa Fluor 750

Applications

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  Recommended    
  Concentration

  Sample

• Intracellular Staining by Flow Cytometry

  0.25-1 µg/10    ⁶ cells

  HEK293 human embryonic kidney cell line transfected with Hemagglutinin/HA-tagged proteins fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # )

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Stability & Storage

  Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: HA Tag

Consecutive histidine residues (usually 6 to 10 in length) are often inserted into the amino acid sequences of recombinant proteins. The resulting His-tagged proteins can be detected or purified by using anti-polyHis antibodies.

• Entrez Gene IDs:

  ABB51961.1 (Viral)

• Alternate Names:

  HA Epitope Tag; HA Tag; HA; Hemagglutinin