• Species Reactivity

  Mouse

• Specificity

  Detects mouse TWEAK R/TNFRSF12 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse (rm) EDA, rmNeogenin, recombinant human TWEAK R, rmOPG, or rmTNF R1 is observed.

• Source

  Monoclonal Rat IgG2a Clone # 314636

• Immunogen

  E. coli-derived recombinant mouse TWEAK R    
  Glu28-Trp79    
  Accession # Q9CR75.1

• Formulation

  Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

• Label

  Alexa Fluor 750

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Flow Cytometry

  0.25-1 µg/10    ⁶ cells

  MS‑1 mouse pancreatic islet endothelial cell line

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: TWEAK R/TNFRSF12

TNF-related weak inducer of apoptosis receptor (TWEAK R) belongs to the TNF receptor superfamily and is designated TNFRSF12. The gene for TWEAK R was originally identified as a fibroblast growth factor-inducible immediate-early response gene Fn14 in mouse NIH 3T3 fibroblasts (1, 2). Mouse TWEAK R cDNA encodes a 129 amino acid (aa) residues type I transmembrane protein with a 27 aa signal peptide, a 53 aa extracellular domain, a 21 aa transmembrane domain and a 28 aa cytoplasmic domain (1‑3). Human and mouse TWEAK R share 82% aa sequence identity. TWEAK R is the smallest member of the TNF receptor superfamily and contains only one cysteine-rich region in its extracellular domain. The TWEAK R cytoplasmic domain contains one TRAF binding motif which binds TRAFs 1, 2, and 3. TWEAK R binds its ligand TWEAK/TNFSF12 with high affinity to initiate a signal transduction cascade that depending upon the cell type, may lead to a variety of cellular responses including cell death by both caspase-dependent apoptosis and cathepsin B-dependent necrosis, cell proliferation, and angiogenesis (2‑6). In newborn mice, TWEAK R is highly expressed in all tissues examined (heart, intestine, kidney, liver, lung and skin) (1). In adult mice, high TWEAK R expression levels are found in the heart and ovary, while lower expression levels are detected in the lung, kidney, skin. Elevated levels of TWEAK R mRNA were found in human or mouse hepatocellular carcinoma specimens, in regenerating mouse liver and in injured rat arteries (2, 3).

• References:

1. Meighan-Mantha, R. et al. (1999) J. Biol. Chem. 274:33166.

2. Feng, S. et al. (2000) Am. J. Pathol. 156:1253.

3. Wiley, S. et al. (2001) Immunity 15:837.

4. Schneider, P. et al. (1999) Eur. J. Immunol. 29:1785.

5. Nakayama, M. et al. (2002) J. Immunol. 168:734.

6. Lynch, C.N. et al. (1999) J. Biol. Chem. 274:8455.

• Long Name:

  TNF-like and Weak Inducer of Apoptosis Receptor

• Entrez Gene IDs:

  51330 (Human); 27279 (Mouse)

• Alternate Names:

  CD266 antigen; CD266; FGF-inducible 14; Fibroblast growth factor-inducible immediate-early response protein 14; Fn14; FN14tumor necrosis factor receptor superfamily member 12A; TNFRSF12; TNFRSF12A; tumor necrosis factor receptor superfamily, member 12A; TWEAK R; TWEAKR; tweak-receptor; type I transmembrane protein Fn14