• Species Reactivity

  Human

• Specificity

  Detects human POT1 in direct ELISAs.

• Source

  Monoclonal Mouse IgG    _2B Clone # 688923

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  E. coli-derived recombinant human POT1    
  Met159-Pro316    
  Accession # Q9NUX5

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Western Blot

  2 µg/mL

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Sterile PBS to a final concentration of 0.5 mg/mL.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: POT1

POT1 (Protection of telomeres protein 1; also POTE1) is a nuclear, 71 kDa member of the telombin family of proteins.  It is ubiquitously expressed, and binds single stranded DNA, TRF2, and TPP1 at the end of chromosomes.  This stabilizes chromosome ends and protects against unwarranted activation of DNA damage programs.  Human POT1 is 634 amino acids (aa) in length.  It contains two N-terminal DNA binding OB regions (aa 1-300) and a C-terminal TPP-1 dimerization domain (aa 301-634).  There are multiple isoform variants.  There is a potential alternate start site at Met132, plus a three aa substitution for aa 43-634, a 24 aa substitution for aa 317-634, a three aa substitution for aa 457-634, and a 16 aa substitution for aa 503-634.  Over aa 159-316, human POT1 shares 71% aa identity with mouse POT1.

• Long Name:

  Protection of Telomeres Protein 1

• Entrez Gene IDs:

  25913 (Human); 101185 (Mouse); 500054 (Rat)

• Alternate Names:

  DKFZp586D211; HPOT1; POT1 protection of telomeres 1 homolog; POT1; POT1-like telomere end-binding protein; protection of telomeres 1 homolog (S. pombe); protection of telomeres protein 1