• Species Reactivity

  Human

• Specificity

  Detects human TRF2 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human TRF1 is observed.

• Source

  Monoclonal Mouse IgG    ₁ Clone # 689524

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  E. coli-derived recombinant human TRF2    
  Gln78-Thr238 (predicted)    
  Accession # Q15554

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Western Blot

  1 µg/mL

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Sterile PBS to a final concentration of 0.5 mg/mL.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TRF-2

TRF2 (telomeric repeat-binding factor 2; also telomeric DNA-binding protein) is a 55-60 kDa, ubiquitously expressed nuclear protein that participates in telomere homeostasis. TRF-2 binds as a dimer to TTAGGG repeats at ends of chromosomes (telomeres), where it blocks inappropriate activation of the ATM/p53 pathway. It also collaborates with TRF1 to promote normal telomere length. Human TRF2 is 500 amino acids (aa) in length. It contains an N-terminal Arg-rich region (aa 13-30), a dimerization domain (aa 46-112), an NLS (aa 329-333), and a DNA binding HTH myb-type domain (aa 442-499). There is one potential alternate start site 42 aa upstream of the standard start site, and one splice form that shows a 13 aa substitution for aa 239-500. Over aa 78-238, human TRF2 shares 96% aa identity with mouse TRF2.

• Long Name:

  Telomeric Repeat Binding Factor 2

• Entrez Gene IDs:

  7014 (Human); 21750 (Mouse); 361403 (Rat)

• Alternate Names:

  Telomeric DNA-binding protein; telomeric repeat binding factor 2; Telomeric repeat binding protein 2; telomeric repeat-binding factor 2; TERF 2; TERF2; TRBF 2; TRBF2; TRF 2; TRF2; TRF-2; TTAGGG repeat binding factor 2; TTAGGG repeat-binding factor 2