• Species Reactivity

  Human

• Specificity

  Detects human Occludin in direct ELISAs.

• Source

  Monoclonal Mouse IgG    ₁ Clone # 690213

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  NS0 mouse myeloma cell line transfected with human Occludin    
  Accession # Q16625

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Immunocytochemistry

  8-25 µg/mL

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Sterile PBS to a final concentration of 0.5 mg/mL.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Occludin

Occludin is an integral membrane protein with an apparent molecular mass of approximately 65 kDa.  It is localized exclusively at tight junctions (TJ) of select epithelial and endothelial cells.  The protein is 522 amino acids (aa) in length and contains a cytoplasmic N-terminus, four transmembrane domains, and a long COOH‑terminal cytoplasmic domain (domain E) that contains 255 aa.  Residues 1-66 make up the first cytoplasmic domain, and that is included in the MARVEL region consisting of aa 57-210.  The MARVEL region is a membrane-associating domain that spans aa 67-243.  Of note, residues 92-131 are glycine and tyrosine rich.  Residues 244-265 constitute the last transmembrane region, with aa 266-522 representing the long cytoplasmic domain termed domain E.  At the TJ, Occludin associates with membrane peripheral protein ZO-1 (220 kDa).  Human Occludin shares 90% aa sequence identity with mouse Occludin.

• Entrez Gene IDs:

  100506658 (Human); 18260 (Mouse); 83497 (Rat)

• Alternate Names:

  BLCPMG; Occludin; OCLN