• Species Reactivity

  Mouse

• Specificity

  Detects mouse EpCAM/TROP-1.

• Source

  Monoclonal Rat IgG2a Clone # G8.8R

• Immunogen

  TE-71 Thymic epithelial cell line

• Formulation

  Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

• Label

  Alexa Fluor 405

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Flow Cytometry

  0.25-1 µg/10    ⁶ cells

  4T1 mouse breast cancer cell line

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Stability & Storage

  Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: EpCAM/TROP1

Epithelial Cellular Adhesion Molecule (EpCAM), also known as EGP314 (Epithelial glycoprotein 314), TACSTD1 (tumor-associated calcium signal transducer 1) and CD326 is a 292 amino acid (aa), 40 kDa transmembrane glycoprotein composed of a 243 aa extracellular domain with two epidermal-growth-factor-like (EGF-like) repeats within the cysteine-rich N-terminal region, a 23 aa transmembrane domain, and a 26 aa cytoplasmic domain. Human and mouse EpCAM share 82% aa sequence identity. During embryonic development, EpCAM is detected in fetal lung, kidney, liver, pancreas, skin, and germ cells. EpCAM has been shown function as a homophilic Ca  ²⁺ independent adhesion molecule (1). Homophilic adhesion via EpCAM requires the interaction of both EGF-like repeats, with the first EGF-like repeat mediating reciprocal interaction between EpCAM molecules on opposing cells, while the second repeat is involved in lateral interaction of EpCAM. Lateral interaction of EpCAM lead to the formation of dimers and tetramers (2). During homophilic adhesion the cytoplasmic tail of EpCAM interacts with the actin cytoskeleton via a direct association alpha -actinin (3).

• References:

1. Litvinow, S.V. et al. (1994) J. Cell Biol. 125:437.

2. Balzar, M. et al. (2001) Mol. Cell. Biol. 21:2570.

3. Balzar, M. et al. (1998) Mol. Cell. Biol. 18:4388.

• Long Name:

  Epithelial Cell Adhesion Molecule

• Entrez Gene IDs:

  4072 (Human); 17075 (Mouse)

• Alternate Names:

  17-1A; 323/A3; ACSTD1; antigen identified by monoclonal AUA1; CD326 antigen; CD326; Cell surface glycoprotein Trop-1; chromosome 4, surface marker (35kD glycoprotein); DIAR5; EGP; EGP-2; EGP314; EGP40; EpCAM; epithelial cell adhesion molecule; Epithelial cell surface antigen; Epithelial glycoprotein 314; Epithelial glycoprotein; ESA; GA733-2; GA733-2EGP34; gp40; hEGP314; HNPCC8; KS 1/4 antigen; KS1/4; KSAHEA125; M1S2; M4S1; M4S1Ly74; Major gastrointestinal tumor-associated protein GA733-2; MIC18MH99; MOC31; TACST-1; TACSTD1; TROP1; TROP1CD326; Tumor-associated calcium signal transducer 1CO-17A