• Species Reactivity

  Human

• Specificity

  Detects human Aminopeptidase N/CD13 in flow cytometry

• Source

  Monoclonal Mouse IgG    ₁ Clone # WM-15

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  Human AML cells    
   

• Formulation

  Supplied in a saline solution containing BSA and Sodium Azide.

• Label

  Alexa Fluor 700

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Flow Cytometry

  5 µL/10    ⁶ cells

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Protect from light.    Do not freeze.    

• 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Aminopeptidase N/CD13

The human ANPEP gene encodes Aminopeptidase N (APN), which is also known as Microsomal Aminopeptidase, Alanyl Aminopeptidase, Aminopeptidase M, CD13, or Membrane Protein p161 (1-3). The deduced amino acid sequence of human APN consists of a short cytoplasmic tail (residues 2 to 8), a transmembrane region (residue 9 to 32), a Ser/Thr rich region and a zinc metalloprotease domain (residues 69 to 966). The amino acid sequence of human APN is 78% and 77% identical to that of rat and mouse, respectively. Widely expressed in many cells, tissues and species, APN cleaves the N-terminal amino acids from bioactive peptides, leading to their inactivation or degradation. The roles of APN in many fields, such as neuroscience, hematopoeitic cells, immune system, angiogenesis, cancer and viral infection, have been reviewed (3).

• References:

1. Olsen, J. et al. (1988) FEBS Lett. 238:307.

2. Look, A.T. et al. (1989) J. Clin. Invest. 83:1299.

3. Turner, A.J. (2004) in Handbook of Proteolytic Enzymes (ed. Barrett, et al.) pp. 289, Academic Press, San Diego.

• Entrez Gene IDs:

  290 (Human); 16790 (Mouse)

• Alternate Names:

  alanyl (membrane) aminopeptidase; Alanyl aminopeptidase; Aminopeptidase M; Aminopeptidase N; ANPEP; AP-M; APN; AP-N; CD13 antigen; CD13; CD13APN; EC 3.4.11; EC 3.4.11.2; gp150; LAP1; Microsomal aminopeptidase; Myeloid plasma membrane glycoprotein CD13; p150; PEPN; PEPNhAPN