详细说明
Species Reactivity
Mouse
Specificity
Detects mouse NAC1 in direct ELISAs.
Source
Recombinant Monoclonal Rabbit IgG Clone # 1243A
Purification
Protein A or G purified from cell culture supernatant
Immunogen
E. coli-derived recombinant mouse NAC1
Val457-Gln514
Accession # Q7TS78Formulation
Supplied as a solution in PBS containing BSA, Glycerol and Sodium Azide. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated
Applications
Recommended
ConcentrationSample
Western Blot
1:1000 dilution
See below
Simple Western
1:100 dilution
See below
Immunohistochemistry
1:70-1:100 dilution
See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. are available in the Technical Information section on our website.
Data Examples
Western Blot | Detection of Mouse NAC1 by Western Blot. Western blot shows lysates of D3 mouse embryonic stem cell line, F9 mouse teratocarcinoma stem cells, and M1 mouse myeloid leukemia cell line. PVDF membrane was probed with 1:1000 dilution of Rabbit Anti-Mouse NAC1 Monoclonal Antibody (Catalog # MAB8375) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # ). A specific band was detected for NAC1 at approximately 65-70 kDa (as indicated). This experiment was conducted under reducing conditions and using . |
Immunohistochemistry | NAC1 in Mouse Embryo. NAC1 was detected in immersion fixed frozen sections of mouse embryo (13 d.p.c.) using Rabbit Anti-Mouse NAC1 Monoclonal Antibody (Catalog # MAB8375) at a 1:80 dilution overnight at 4 °C. Tissue was stained using the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # ) and counterstained with hematoxylin (blue). Specific staining was localized to developing primary sensory neurons in dorsal root ganglia. View our protocol for . |
Simple Western | Detection of Mouse NAC1 by Simple WesternTM. Simple Western lane view shows lysate of D3 mouse embryonic stem cell line, loaded at 0.2 mg/mL. A specific band was detected for NAC1 at approximately 69 kDa (as indicated) using 1 µg/mL of Rabbit Anti-Mouse NAC1 Monoclonal Antibody (Catalog # MAB8375). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. |
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 °C, as supplied.
1 month, 2 to 8 °C under sterile conditions after opening.
6 months, -20 to -70 °C under sterile conditions after opening.
Background: NAC1
NAC1 (Nucleus Accumbens-associated Protein 1) also known as NACC1, is a 527 amino acid (aa) BTB/POZ domain containing protein. Human NAC1 shares 86% and 85% aa sequence identity with mouse and rat NAC1, respectively. It has been shown to have a role in transcriptional regulation in both pluripotent stem cells and in the brain. Additionally, NAC1 expression is upregulated in many cancers and this expression has been suggested to correlate with increased aggressiveness in some cancers.
Long Name:
Nucleus Accumbens Associated 1
Entrez Gene IDs:
112939 (Human); 66830 (Mouse); 171454 (Rat)
Alternate Names:
BEND8; BTB (POZ) domain containing 14B; BTB/POZ domain-containing protein 14B; BTBD14B; BTBD30; NAC1; NAC1BEN domain containing 8; NAC-1FLJ37383; NACC1; nucleus accumbens associated 1, BEN and BTB (POZ) domain containing; nucleus accumbens-associated protein 1; transcriptional repressor NAC1
粤公网安备44196802000105号
400-6226-992