• Species Reactivity

  Human

• Specificity

  Detects human CLEC10A/CD301 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human ASGPR1 or recombinant mouse MGL1 is observed.

• Source

  Monoclonal Mouse IgG2b Clone # 744812

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant human CLEC10A/CD301    
  Gln61-His316    
  Accession # Q8IUN9

• Formulation

  Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

• Label

  Alexa Fluor 750

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Flow Cytometry

  0.25-1 µg/10    ⁶ cells

  Immature human dendritic cells

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CLEC10A/CD301

CLEC10A, also known as macrophage galactose/N-acetyl-galactosamine (GalNAc) specific lectin (MGL), CD301, DC-ASGPR, and HML, is a 40 kDa type II transmembrane glycoprotein that belongs to the C-type lectin family (1). Human and rat carry a single gene for CLEC10A/MGL, while mouse has two closely related MGL1 and MGL2 genes. Human CLEC10A/MGL consists of a 39 amino acid (aa) cytoplasmic region, a 21 aa transmembrane segment and a 256 aa extracellular domain (ECD) with one carbohydrate recognition domain (CRD) and a neck region (2). Within the CRD, human CLEC10A/MGL shares 64%-70% aa sequence identity with mouse MGL1, mouse MGL2, and rat MGL. Alternate splicing generates multiple isoforms of human CLEC10A/MGL with 27 aa, 3 aa, and/or 4 aa deletions within the ECD (3, 4). CLEC10A/MGL is expressed on immature myleloid dendritic cells and alternatively activated (tolerogenic) macrophages and is upregulated by the immunosuppressant dexamethasone (3-7). CLEC10A/MGL selectively binds and internalizes terminal nonsialylated alpha - or beta -linked GalNAc moieties on O-linked carbohydrates, including the Tn carcinoma antigen (2-4, 8, 9). Similar ligand preference is exhibited by mouse MGL2 but not MGL1 (10). CLEC10A/MGL expressed on tolerogenic dendritic cells binds carbohydrate determinants on CD45 (RA, RB, and RC but not RO isoforms) expressed by T, NK, and B cells (6). This interaction inhibits effector T cell activation and induces their apoptosis (6). CLEC10A/MGL also binds the GP envelope glycoprotein on Marburg and Ebola viruses and enhances viral entry and infectivity (11).

• References:

1. Zelensky, A.N. and J.E. Gready (2005) FEBS J. 272:6179.

2. Suzuki, N. et al. (1996) J. Immunol. 156:128. 

3. Higashi, N. et al. (2002) J. Biol. Chem. 277:20686. 

4. Valladeau, J. et al. (2001) J. Immunol. 167:5767.

5. van Vliet, S.J. et al. (2006) Immunobiology 211:577. 

6. van Vliet, S.J. et al. (2006) Nat. Immunol. 7:1200.

7. Raes, G. et al. (2005) J. Leukoc. Biol. 77:321.

8. van Vliet, S.J. et al. (2005) Int. Immunol. 17:661.

9. Higashi, N. et al. (2002) Int. Immunol. 14:545.

10. Tsuiji, M. et al. (2002) J. Biol. Chem. 277:28892.

11. Takada, A. et al. (2004) J. Virol. 78:2943.

• Long Name:

  Macrophage Galactose N-Acetyl-Galactosamine Specific Lectin 1

• Entrez Gene IDs:

  10462 (Human)

• Alternate Names:

  CD301 antigen; CD301; CD301C-type lectin domain family 10 member A; CLEC10A; CLECSF13; CLECSF14; CLECSF14macrophage C-type lectin; C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamilymember 14 (macrophage-derived); C-type lectin domain family 10, member A; C-type lectin superfamily member 14; HML2; HMLC-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamilymember 13 (macrophage-derived); macrophage lectin 2 (calcium dependent); Macrophage lectin 2; MGL