• Species Reactivity

  Mouse

• Specificity

  Detects mouse CD34 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human, rat, or porcine CD34 is observed.

• Source

  Monoclonal Rat IgG2a Clone # 700011

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant mouse CD34    
  Thr35-Thr287    
  Accession # Q64314

• Formulation

  Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

• Label

  Alexa Fluor 750

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Flow Cytometry

  0.25-1 µg/10    ⁶ cells

  bEnd.3 mouse endothelioma cell line

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CD34

CD34 is a 100-105 kDa member of the CD34/podocalyxin family of molecules. It is a sialomucin type glycoprotein, and presents carbohydrate to selectins during cell migration. CD34 is found on mast cells, eosinophils, vascular endothelial cells, CD34  ⁺ stem cells and renal mesangial cells. Mature mouse CD34 is a 348 amino acid (aa) type I transmembrane protein (aa 35-382). It contains a 253 aa extracellular region (aa 35-287), and a 74 aa cytoplasmic domain that undergoes Tyr phosphorylation. There is one 90 kDa splice variant that shows a four aa substitution for aa 322-382. Mouse CD34 also is likely to undergo membrane cleavage that will generate a 54 kDa soluble form. Over aa 35-287, mouse CD34 shares 57% and 82% aa identity with human and rat CD34, respectively.  

• Entrez Gene IDs:

  947 (Human); 12490 (Mouse); 305081 (Rat); 397160 (Porcine); 415130 (Canine)

• Alternate Names:

  CD34 antigenhematopoietic progenitor cell antigen CD34; CD34 molecule; CD34; HPCA1