• Species Reactivity

  Cotton Rat

• Specificity

  Detects cotton rat CD4 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant CD4 from human, mouse, rat, cat, or dog is observed.

• Source

  Monoclonal Mouse IgG2b Clone # 695542

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant cotton rat CD4    
  Lys26-Pro393

• Formulation

  Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

• Label

  Alexa Fluor 750

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Flow Cytometry

  0.25-1 µg/10    ⁶ cells

  Cotton rat splenocytes

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CD4

CD4 is an approximately 55 kDa type I transmembrane glycoprotein that is expressed predominantly on thymocytes and the Th1, Th2, Th17, Tfh, and Treg subsets of mature T lymphocytes. In human, CD4 is additionally expressed on macrophages, neutrophils, monocytes, NK cells, and neurons and glial cells in the brain. CD4 binding to MHC class II molecules on antigen presenting cells contributes to the formation of the immunological synapse. CD4 also functions as a chemotactic receptor for IL-16 and, in human, as a coreceptor for the gp120 surface glycoprotein of HIV-1.

• Entrez Gene IDs:

  920 (Human); 12504 (Mouse); 24932 (Rat); 403931 (Canine); 493775 (Feline)

• Alternate Names:

  CD4 antigen (p55); CD4 antigen; CD4 molecule; CD4 receptor; CD4; CD4mut; T-cell surface antigen T4/Leu-3; T-cell surface glycoprotein CD4