• Species Reactivity

  Human

• Specificity

  Detects human CD160 in direct ELISAs. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse CD160 is observed.

• Source

  Monoclonal Mouse IgG2b Clone # 688327

• Immunogen

  Chinese hamster ovary cell line CHO-derived recombinant human CD160    
  Ile27-Ser159    
  Accession # O95971

• Formulation

  Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

• Label

  Alexa Fluor 750

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Flow Cytometry

  0.25-1 µg/10    ⁶ cells

  Human peripheral blood lymphocytes

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CD160

CD160 (also BY55) is a 27 kDa member of the immunoglobulin superfamily of molecules.  It is expressed on select hematopoietic cell types, including CD56  ^dim CD16  ⁺ cytotoxic NK cells, CD8  ⁺ CD28  ^- effector T cells, δ/ gamma T cells, and restricted CD4  ⁺ T cells.  It is a receptor for HLA-C molecules, and its engagement induces CD160  ⁺ NK cells to both secrete IFN-gamma plus TNF-alpha and initiate a cytotoxic program.  Human CD160 was originally identified as a 155 amino acid (aa) proprotein (aa 27‑181).  It contains a 132 aa mature region (aa 27-159) and a C-terminal prosegment that is cleaved to create a GPI linkage. The mature region possesses one  
V‑type Ig-like domain (aa 27-122).  CD160 is found as a soluble, disulfide-linked 80 kDa multimer (likely trimer) that is generated by proteolysis of the GPI-linked form. This 80 kDa form, plus others, are highly resistant to reduction. There is also a 100-110 kDa multimeric transmembrane (TM) form that is associated with activated NK cells. It contains a 55 aa substitution for Gly180-Leu181, and shows a 20 aa TM segment between aa 163-182.  The TM form appears to have a splice variant that lacks aa 25-133.  Over aa 27-159, human CD160 shares 62% aa identity with mouse CD160.  

• Entrez Gene IDs:

  11126 (Human); 54215 (Mouse); 502585 (Rat)

• Alternate Names:

  BY55; BY55FLJ46513; CD160 antigenimmunoglobulin superfamily member; CD160 molecule; CD160; Natural killer cell receptor BY55; NK1; NK28