• Species Reactivity

  Human

• Specificity

  Detects human CD34 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse, rat, or porcine CD34 is observed.

• Source

  Monoclonal Mouse IgG1 Clone # 756510

• Immunogen

  Chinese hamster ovary cell line CHO-derived recombinant human CD34    
  Ser32-Thr290    
  Accession # P28906

• Formulation

  Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

• Label

  Alexa Fluor 750

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Flow Cytometry

  0.25-1 µg/10    ⁶ cells

  KG-1a acute myelogenous leukemia cell line

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CD34

The CD34 family of cell surface transmembrane proteins includes the hematopoietic progenitor cell antigen CD34, Podocalyxin, and Endoglycan. This single-pass sialomucin‑like transmembrane protein is heavily glycosylated and phosphorylated by Protein kinase C (PKC). CD34 is a 115 kDa glycoprotein found on multipotent precursors, bone marrow stromal cells, embryonic fibroblasts, vascular endothelia, as well as some populations of mesenchymal stem cells, and tumor cell lines. CD34 is involved in the adhesion of stem cells to the bone marrow extracellular matrix or to stromal cells.

• Entrez Gene IDs:

  947 (Human); 12490 (Mouse); 305081 (Rat); 397160 (Porcine); 415130 (Canine)

• Alternate Names:

  CD34 antigenhematopoietic progenitor cell antigen CD34; CD34 molecule; CD34; HPCA1