• Species Reactivity

  Human

• Specificity

  Detects human PSGL-1/CD162 in direct ELISAs.

• Source

  Monoclonal Mouse IgG2a Clone # 688101

• Immunogen

  Chinese hamster ovary cell line CHO-derived recombinant human PSGL-1/CD162    
  Gln42-Gly295    
  Accession # NP_002997

• Formulation

  Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

• Label

  Alexa Fluor 750

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Flow Cytometry

  0.25-1 µg/10    ⁶ cells

  Human peripheral blood monocytes

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: PSGL-1/CD162

Human PSGL-1 (P-Selectin Glycoprotein Ligand-1; also CD162), is a 120 kDa mucin-type glycoprotein that plays a key role in leukocyte adhesion (1-3). It is synthesized as a 412 amino acid (aa) preproprecursor that contains a 17 aa signal sequence, a 24 aa propeptide, a 279 aa extracellular domain (ECD), a 21 aa transmembrane segment and a 71 aa cytoplasmic region (4, 5). Following cleavage of the pre- and prosegments, it is expressed as a 240 kDa disulfide-linked homodimer. The extreme N-terminus (aa 1-16 of the mature molecule) contains one threonine (aa 16) and three tyrosines (aa 5, 7, and 10) that are involved in ligand binding. The Thr residue allows for O-linked glycosylation in the form of a core-2 structure (GalNAc-Gal) linked in a beta 1,6 bond to a sialylated Lewis X motif (GlcNAc linked to both Fuc and Gal with a terminal sialic acid residue) (1, 2, 5, 6, 7). The three tyrosine residues allow for sulfation (8, 9). When binding to P-selectin, Tyr sulfation and glycosylation are essential. Tyr7 provides the most efficient sulfate moiety, while Fuc and sialic acid are essentially mandatory (7). When binding to E-selectin, only carbohydrate is needed, while both carbohydrate and Tyr10 are used for L-selectin binding (6, 8). There are 16 decameric aa repeats in the ECD of the longform of PSGL-1. This form is referred to as the A allele, and represents 65 - 80% of the population. Alleles B and C show deletions of decameric repeats #2 (aa 132-141) plus #9 and 10 (aa 222-241), respectively. Shorter forms may show weaker binding to P-selectin (9, 10). Soluble forms of PSGL-1 are also known. Neutrophil elastase will cleave somewhere within repeats #5-9, while cathepsin G cleaves after Tyr7 (11). The loss of Tyr5 and 7 should impact binding affinity. PSGL-1 is found on virtually all leukocytes and macrophages/DC’s (1). Although there is similarity in the organization of the ECD between species, there is little aa identity. Human PSGL-1 ECD shares 51%, 52% and 43% aa sequence identity with equine, canine and mouse ECD, respectively.

• References:

1. Yang, J. et al. (1999) Thromb. Haemost. 81:1.

2. Cummings, R.D. (1999) Braz. J. Med. Biol. Res. 32:519.

3. McEver, R.P. and R.D. Cummings (1997) J. Clin. Invest. 100:485.

4. Sako, D. et al. (1993) Cell 75:1179.

5. Veldman, G.M. et al. (1995) J. Biol. Chem. 270:16470.

6. Bernimoulin, M.P. et al. (2003) J. Biol. Chem. 278:37.

7. Leppanen, A. et al. (2000) J. Biol. Chem. 275:39569.

8. Sako, D. et al. (1995) Cell 83:323.

9. Afshar-Kharghan, V. et al. (2001) Blood 97:3306.

10. Lozano, M.L. et al. (2001) Br. J. Haematol. 115:969.

11. Gardiner, E.E. et al. (2001) Blood 98:1440.

• Long Name:

  P-Selectin Glycoprotein Ligand 1

• Entrez Gene IDs:

  6404 (Human); 20345 (Mouse); 363930 (Rat)

• Alternate Names:

  CD162 antigen; CD162; P-selectin glycoprotein ligand 1; PSGL1; PSGL-1; PSGL-1cutaneous lymphocyte-associated associated antigen; selectin P ligandCLA; SELPLG